通过免疫电泳试验评估完整和纯化的包虫囊液抗原在人类包虫病诊断中的作用。

Evaluation of whole and purified hydatid fluid antigens in the diagnosis of human hydatidosis by the immunoelectrophoresis test.

作者信息

Varela-Díaz V M, Guisantes J A, Ricardes M I, Yarzábal L A, Coltorti E A

出版信息

Am J Trop Med Hyg. 1975 Mar;24(2):298-303. doi: 10.4269/ajtmh.1975.24.298.

DOI:10.4269/ajtmh.1975.24.298

Abstract

Whole sheep hydatid cyst fluid (WHF) and purified hydatid fluid antigens (PHF) were evaluated in the immunoelectrophoresis (IEP) test for the diagnosis of human hydatid disease. A higher sensitivity was obtained using the PHF than with the WHF antigen but false positive results were observed with the former in 22.4% of the 102 non-hydatid sera examined. Using the presence of arc 5 as the criterion of positivity however, no false positives were observed in non-hydatid sera with the WHF antigen. It is postulated that the number of bands other than arc 5 may be of value in the diagnosis of hydatid disease by the IEP test, in cases where this diagnostic arc is absent.

摘要

对全羊包虫囊肿液（WHF）和纯化的包虫液抗原（PHF）进行了免疫电泳（IEP）试验，以诊断人类包虫病。与WHF抗原相比，使用PHF获得了更高的灵敏度，但在检测的102份非包虫血清中，有22.4%的血清出现了前者的假阳性结果。然而，以弧5的存在作为阳性标准，使用WHF抗原时在非包虫血清中未观察到假阳性。据推测，在没有这种诊断性弧的情况下，IEP试验中除弧5以外的条带数量可能对包虫病的诊断有价值。