用双向扩散试验检测抗细粒棘球绦虫5号抗原的抗体

Detection of antibodies against Echinococcus granulosus arc 5 antigens by double diffusion test.

作者信息

Coltorti E A, Varela-Díaz V M

出版信息

Trans R Soc Trop Med Hyg. 1978;72(3):226-9. doi: 10.1016/0035-9203(78)90198-0.

Abstract

This report deals with an evaluation of a double diffusion (DD5) test which employs a control antiserum against Echinococcus granulosus arc 5 antigens to recognize arc 5-positive sera by a reaction of identity. The DD5 test was more sensitive than the immuno-electrophoresis (IEP5) test based on the same positivity criterion and was equally specific for the immunodiagnosis of human hydatidosis. Its greater simplicity suggest its application as a substitute for the IEP5 test in diagnosis, particularly areas. The application of both DD5 and latex agglutination tests is recommended for hydatid immunodiagnosis on the basis of the present findings.

摘要

本报告涉及对一种双向扩散（DD5）试验的评估，该试验采用针对细粒棘球绦虫5区抗原的对照抗血清，通过同一性反应识别5区阳性血清。基于相同的阳性标准，DD5试验比免疫电泳（IEP5）试验更敏感，并且在人类包虫病的免疫诊断中具有同等特异性。其更高的简易性表明它可作为IEP5试验的替代方法用于诊断，尤其是在某些地区。根据目前的研究结果，建议将DD5试验和乳胶凝集试验都用于包虫病的免疫诊断。