Vidal K, Labéta M O, Schiffrin E J, Donnet-Hughes A
Nestlé Research Center, Vers-Chez-Les-Blanc, Lausanne, Switzerland.
Acta Odontol Scand. 2001 Oct;59(5):330-4. doi: 10.1080/000163501750541219.
Immune factors secreted in milk are important for health in the neonatal gut. We have detected the bacterial pattern recognition receptor, soluble CD14 (sCD14) in human breast milk at different times during lactation. The molecule occurs in a single form in milk, in contrast to human serum, in which there are two isoforms. Produced by mammary epithelial cells, milk sCD14 mediates secretion of innate immune response molecules such as interleukin-8, tumor necrosis factor-alpha, and epithelial neutrophil activator-78 by CD14-negative intestinal epithelial cells exposed to lipopolysaccharide (LPS) or bacteria. Although present at low concentrations in milk, LPS-binding protein may be implicated in the biological effects observed. Our findings support the premise that milk sCD14 acts as a 'sentinel' molecule and immune modulator in homeostasis and in the defense of the neonatal intestine. In so doing, it may prevent the immune and inflammatory conditions of the gut to which non-breastfed infants are predisposed.
母乳中分泌的免疫因子对新生儿肠道健康至关重要。我们在哺乳期不同时间检测了人母乳中的细菌模式识别受体——可溶性CD14（sCD14）。与人类血清中存在两种同种型不同，该分子在母乳中以单一形式存在。母乳sCD14由乳腺上皮细胞产生，可介导暴露于脂多糖（LPS）或细菌的CD14阴性肠上皮细胞分泌白细胞介素-8、肿瘤坏死因子-α和上皮中性粒细胞激活因子-78等先天性免疫反应分子。尽管LPS结合蛋白在母乳中的浓度较低，但其可能与所观察到的生物学效应有关。我们的研究结果支持这样一个前提，即母乳sCD14在新生儿肠道的稳态和防御中充当“哨兵”分子和免疫调节剂。这样做可能会预防非母乳喂养婴儿易患的肠道免疫和炎症性疾病。
