Arranz O, Ara J, Rodriguez R, Quintó L, Font J, Mirapeix E, Darnell A
Nephrology Service, Hospital Clinic, Instituto de Investigaciones Biomédicas (IDIBAPS), Universidad de Barcelona, Spain.
Clin Nephrol. 2001 Oct;56(4):295-301.
A total of 118 sera from 11 patients with anti-neutrophil cytoplasmic antibodies against proteinase-3- (PR3-ANCA) associated vasculitis were retrospectively screened by anti-PR3 capture and anti-PR3 direct ELISA tests. We studied the relationship between capture and direct ELISA scores and the clinical activity of PR3-ANCA-associated vasculitis patients during follow-up. We also studied the ability of the anti-PR3 capture ELISA to detect positive values of PR3-ANCA in clinical vasculitis relapses. Only capture ELISA presented a significant relationship (p < 0.05) with clinical activity of PR3-ANCA-associated vasculitis patients over time. Capture ELISA appears to be a reliable method for detecting clinical relapses in this group of patients. Our results indicate that the new capture ELISA test is more effective than direct ELISA in the follow-up of patients with PR3-ANCA-associated vasculitis and in the detection of relapses.
通过抗蛋白酶3-抗中性粒细胞胞浆抗体（PR3-ANCA）捕获法和抗PR3直接酶联免疫吸附测定（ELISA）试验，对11例PR3-ANCA相关性血管炎患者的118份血清进行了回顾性筛查。我们研究了捕获法和直接ELISA评分与PR3-ANCA相关性血管炎患者随访期间临床活动度之间的关系。我们还研究了抗PR3捕获ELISA在临床血管炎复发时检测PR3-ANCA阳性值的能力。随着时间的推移，只有捕获ELISA与PR3-ANCA相关性血管炎患者的临床活动度呈现显著相关性（p<0.05）。捕获ELISA似乎是检测该组患者临床复发的可靠方法。我们的结果表明，在PR3-ANCA相关性血管炎患者的随访及复发检测中，新的捕获ELISA试验比直接ELISA更有效。
