聚腺苷酸结合蛋白在酵母细胞质提取物中调节mRNA去腺苷酸化和脱帽过程。
Poly(A)-binding proteins regulate both mRNA deadenylation and decapping in yeast cytoplasmic extracts.
作者信息
Wilusz C J, Gao M, Jones C L, Wilusz J, Peltz S W
机构信息
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854, USA.
出版信息
RNA. 2001 Oct;7(10):1416-24.
Abstract
The pathway of mRNA degradation has been extensively studied in the yeast, Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation-dependent mechanism. Although several of the factors required for mRNA decay have been identified, the regulation and precise roles of many of the proteins involved remains unclear. We have developed an in vitro system that recapitulates both the deadenylation and the decapping steps of mRNA decay. Furthermore, both deadenylation and decapping are inhibited by poly(A) binding proteins in our assay. Our system has allowed us to separate the decay process from translation and we have shown that the poly(A) tail is capable of inhibiting decapping in an eIF4E-independent manner. Our in vitro system should prove invaluable in dissecting the mechanisms of mRNA turnover.
摘要
信使核糖核酸（mRNA）降解途径已在酿酒酵母中得到广泛研究，现在很清楚的是，许多mRNA通过依赖去腺苷酸化的机制降解。虽然已经鉴定出mRNA降解所需的几个因子，但许多相关蛋白质的调控和精确作用仍不清楚。我们开发了一种体外系统，该系统概括了mRNA降解的去腺苷酸化和脱帽步骤。此外，在我们的实验中，去腺苷酸化和脱帽都受到聚腺苷酸结合蛋白的抑制。我们系统使我们能够将降解过程与翻译分开，并且我们已经表明，聚腺苷酸尾能够以不依赖真核起始因子4E（eIF4E）的方式抑制脱帽。我们的体外系统在剖析mRNA周转机制方面应被证明具有极高价值。
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本文引用的文献
1
The cap-to-tail guide to mRNA turnover.mRNA 周转的从头到尾指南。
Nat Rev Mol Cell Biol. 2001 Apr;2(4):237-46. doi: 10.1038/35067025.
2
The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae.与转录因子相关的Ccr4和Caf1蛋白是酿酒酵母中主要的细胞质mRNA脱腺苷酸化酶的组成部分。
Cell. 2001 Feb 9;104(3):377-86. doi: 10.1016/s0092-8674(01)00225-2.
3
A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements.HeLa细胞质提取物中的一种新型mRNA去帽活性受富含AU元件的调控。
EMBO J. 2001 Mar 1;20(5):1134-43. doi: 10.1093/emboj/20.5.1134.
4
mRNA decapping in yeast requires dissociation of the cap binding protein, eukaryotic translation initiation factor 4E.酵母中的mRNA去帽需要帽结合蛋白（真核生物翻译起始因子4E）的解离。
Mol Cell Biol. 2000 Nov;20(21):7933-42. doi: 10.1128/MCB.20.21.7933-7942.2000.
5
The eukaryotic mRNA decapping protein Dcp1 interacts physically and functionally with the eIF4F translation initiation complex.真核生物的mRNA去帽蛋白Dcp1在物理和功能上与eIF4F翻译起始复合物相互作用。
EMBO J. 2000 Aug 15;19(16):4372-82. doi: 10.1093/emboj/19.16.4372.
6
The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p.两种蛋白质Pat1p（Mrt1p）和Spb8p在体内相互作用，是mRNA降解所必需的，并且在功能上与Pab1p相关联。
Mol Cell Biol. 2000 Aug;20(16):5939-46. doi: 10.1128/MCB.20.16.5939-5946.2000.
7
Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro.聚腺苷酸特异性核糖核酸酶与5'帽之间的相互作用影响体外mRNA去腺苷酸化速率。
Mol Cell. 2000 Mar;5(3):479-88. doi: 10.1016/s1097-2765(00)80442-6.
8
Inhibition of mRNA turnover in yeast by an xrn1 mutation enhances the requirement for eIF4E binding to eIF4G and for proper capping of transcripts by Ceg1p.xrn1突变对酵母中mRNA周转的抑制增强了eIF4E与eIF4G结合以及Ceg1p对转录本进行正确加帽的需求。
Genetics. 2000 May;155(1):31-42. doi: 10.1093/genetics/155.1.31.
9
Yeast Sm-like proteins function in mRNA decapping and decay.酵母Sm样蛋白在mRNA脱帽和降解过程中发挥作用。
Nature. 2000 Mar 30;404(6777):515-8. doi: 10.1038/35006676.
10
Cap-dependent deadenylation of mRNA.mRNA的帽依赖性去腺苷酸化
EMBO J. 2000 Mar 1;19(5):1079-86. doi: 10.1093/emboj/19.5.1079.
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