Yamagaki T, Nakanishi H
Department of Chemistry, School of Science, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Proteomics. 2001 Feb;1(2):329-39. doi: 10.1002/1615-9861(200102)1:2<329::AID-PROT329>3.0.CO;2-K.
The various kinds of oligosaccharides were analyzed by using post-source decay (PSD) fragmentation method of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Since the curved field reflectron MALDI-MS can record all fragment ions in a single measurement, we can discuss the fragment ion intensity in PSD mass spectrum more accurately. The intensities of the PSD fragment ions indicate the fine structure of the saccharide chains. The type of glycosyl linkages could be determined by the ion intensity analysis, and the stereo isomers of monosaccharides were distinguished by the MALDI-PSD fragment ion analysis. The linkage isomers and structural isomers were also distinguished by this method. The ion intensity analysis of curved-field reflectron MALDI-MS could be a powerful tool for glycosylation analysis.
利用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的源后衰变（PSD）碎裂方法对各种寡糖进行了分析。由于弯曲场反射式MALDI-MS能够在单次测量中记录所有碎片离子，因此我们可以更准确地讨论PSD质谱图中的碎片离子强度。PSD碎片离子的强度表明了糖链的精细结构。通过离子强度分析可以确定糖基连接的类型，通过MALDI-PSD碎片离子分析可以区分单糖的立体异构体。该方法还可以区分连接异构体和结构异构体。弯曲场反射式MALDI-MS的离子强度分析可能是糖基化分析的有力工具。
