Mukherjee T, Lakhotia S C
Chromosoma. 1979 Sep 1;74(1):75-82. doi: 10.1007/BF00344484.
The autoradiographic patterns of 3H-uridine labelling of the major temperature shock puff sites and the chromocentric (beta-)heterochromatin in heat shocked (37 degrees C) salivary glands of Drosophila melanogaster have been studied. It is seen that in response to the heat shock treatment, four of the major temperature shock puffs (63BC, 87A, 87C and 95D) show a correlated level of 3H-uridine incorporation in a given nucleus. However, although the mean grain density on 93D puff is maximum in the heat shocked preparations, in individual nuclei this puff is labelled to a widely varying level and this variation in its labelling is statistically not correlated to the labelling of the other four temperature shock puffs in a nucleus. The chromocentric beta-heterochromatin, which has been shown in several earlier studies to hybridize with temperature shock RNA fractions, is seen to be totally inactive in transcription after the heat shock.
对黑腹果蝇热激（37摄氏度）唾液腺中主要温度休克胀泡位点和着丝粒（β-）异染色质的3H-尿苷标记的放射自显影模式进行了研究。可以看到，响应热激处理，四个主要温度休克胀泡（63BC、87A、87C和95D）在给定细胞核中显示出相关的3H-尿苷掺入水平。然而，尽管在热激制剂中93D胀泡上的平均颗粒密度最大，但在单个细胞核中，这个胀泡的标记水平差异很大，并且其标记的这种变化在统计学上与细胞核中其他四个温度休克胀泡的标记不相关。着丝粒β-异染色质在早期的几项研究中已显示与温度休克RNA组分杂交，在热激后其转录完全无活性。
