源自富含微卫星的染色体特异性文库的兔1号染色体标记的定位。

Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library.

作者信息

Korstanje R, Gillissen G F, den Bieman M G, Versteeg S A, van Oost B, Fox R R, van Lith H A, van Zutphen L F

机构信息

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

出版信息

Anim Genet. 2001 Oct;32(5):308-12. doi: 10.1046/j.1365-2052.2001.00783.x.

DOI:10.1046/j.1365-2052.2001.00783.x

PMID:11683719

Abstract

A genomic DNA library was produced from flow-sorted rabbit chromosome 1 and enriched for fragments containing CA-repeats. Clones containing CA-repeats were identified and primers for amplification of the microsatellite were developed after sequencing the clone. The degree of polymorphism was tested in rabbits from different breeds. This approach identified 12 microsatellite markers which could be used for studying linkage relationships in the progeny of an F(2)-intercross: (AX/JUxIIIVO/JU) F(2), and two backcrosses: (OS/JxX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosome 1.

摘要

从流式分选的兔1号染色体构建了基因组DNA文库，并富集了含CA重复序列的片段。鉴定出含CA重复序列的克隆，并在对克隆进行测序后开发了用于扩增微卫星的引物。在不同品种的兔子中测试了多态性程度。该方法鉴定出12个微卫星标记，可用于研究F(2)杂交后代：(AX/JUxIIIVO/JU) F(2)，以及两个回交后代：(OS/JxX/J)X/J和(WH/JxX/J)X/J中的连锁关系。其中7个标记定位在1号染色体上。