Ravanat J L, Douki T, Cadet J
Laboratoire Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique, UMR 5046, Département de Recherche Fondamentale sur la Matière Condensée, CEA Grenoble, 17 Avenue des Martyrs, F-38054 Grenoble Cedex 9, France.
J Photochem Photobiol B. 2001 Oct;63(1-3):88-102. doi: 10.1016/s1011-1344(01)00206-8.
In this survey, emphasis was placed on the main photoreactions of nucleic acid components, involving both direct and indirect effects. The main UVB- and UVA-induced DNA photoproducts, together with the mechanisms of their formation, are described. Information on the photoproduct distribution within cellular DNA is also provided, taking into account the limitations of the different analytical methods applied to monitor the formation of the DNA damage. Thus, the formation of the main DNA dimeric pyrimidine lesions produced by direct absorption of UVB photons was assessed using a powerful HPLC-tandem mass spectrometry assay. In addition, it was found that UVA photooxidation damage mostly involves the guanine residues of cellular DNA as the result of singlet oxygen generation by still unknown endogenous photosensitizers.
在本次调查中，重点关注了核酸成分的主要光反应，包括直接和间接效应。描述了主要的紫外线B（UVB）和紫外线A（UVA）诱导的DNA光产物及其形成机制。还提供了关于细胞DNA内光产物分布的信息，同时考虑到用于监测DNA损伤形成的不同分析方法的局限性。因此，使用强大的高效液相色谱-串联质谱分析法评估了由UVB光子直接吸收产生的主要DNA二聚嘧啶损伤的形成。此外，还发现UVA光氧化损伤主要涉及细胞DNA的鸟嘌呤残基，这是由尚不清楚的内源性光敏剂产生单线态氧的结果。
