一种涉及Csk SH3结构域及其天然配体的新型特异性相互作用。
A novel, specific interaction involving the Csk SH3 domain and its natural ligand.
作者信息
Ghose R, Shekhtman A, Goger M J, Ji H, Cowburn D
机构信息
The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399, USA.
出版信息
Nat Struct Biol. 2001 Nov;8(11):998-1004. doi: 10.1038/nsb1101-998.
Abstract
C-terminal Src kinase (Csk) takes part in a highly specific, high affinity interaction via its Src homology 3 (SH3) domain with the proline-enriched tyrosine phosphatase PEP in hematopoietic cells. The solution structure of the Csk-SH3 domain in complex with a 25-residue peptide from the Pro/Glu/Ser/Thr-rich (PEST) domain of PEP reveals the basis for this specific peptide recognition motif involving an SH3 domain. Three residues, Ala 40, Thr 42 and Lys 43, in the SH3 domain of Csk specifically recognize two hydrophobic residues, Ile 625 and Val 626, in the proline-rich sequence of the PEST domain of PEP. These two residues are C-terminal to the conventional proline-rich SH3 domain recognition sequence of PEP. This interaction is required in addition to the classic polyproline helix (PPII) recognition by the Csk-SH3 domain for the association between Csk and PEP in vivo. NMR relaxation analysis suggests that Csk-SH3 has different dynamic properties in the various subsites important for peptide recognition.
摘要
C 端 Src 激酶（Csk）通过其Src 同源 3（SH3）结构域与造血细胞中富含脯氨酸的酪氨酸磷酸酶 PEP 进行高度特异性、高亲和力的相互作用。Csk-SH3 结构域与来自 PEP 的富含脯氨酸/谷氨酸/丝氨酸/苏氨酸（PEST）结构域的 25 个残基肽形成的复合物的溶液结构揭示了这种涉及 SH3 结构域的特异性肽识别基序的基础。Csk 的 SH3 结构域中的三个残基，即 Ala 40、Thr 42 和 Lys 43，特异性识别 PEP 的 PEST 结构域富含脯氨酸序列中的两个疏水残基，Ile 625 和 Val 626。这两个残基位于 PEP 传统富含脯氨酸的 SH3 结构域识别序列的 C 端。除了 Csk-SH3 结构域对经典多聚脯氨酸螺旋（PPII）的识别外，这种相互作用对于体内 Csk 和 PEP 之间的结合也是必需的。核磁共振弛豫分析表明，Csk-SH3 在对肽识别重要的各个亚位点具有不同的动力学性质。
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本文引用的文献
1
A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams. 1991.一种使用等高线图自动计算机分析在二维、三维和四维光谱中进行峰挑选的常识性方法。1991年。
J Magn Reson. 2011 Dec;213(2):357-63. doi: 10.1016/j.jmr.2011.09.007.
2
Determination of the rotational diffusion tensor of macromolecules in solution from nmr relaxation data with a combination of exact and approximate methods--application to the determination of interdomain orientation in multidomain proteins.结合精确方法和近似方法，根据核磁共振弛豫数据测定溶液中大分子的旋转扩散张量——应用于多结构域蛋白质结构域间取向的测定
J Magn Reson. 2001 Apr;149(2):204-17. doi: 10.1006/jmre.2001.2295.
3
SH3 domains: complexity in moderation.SH3结构域：适度的复杂性
J Cell Sci. 2001 Apr;114(Pt 7):1253-63. doi: 10.1242/jcs.114.7.1253.
4
Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor.环磷酸腺苷依赖性蛋白激酶对羧基末端Src激酶（Csk）的激活会抑制通过T细胞受体的信号传导。
J Exp Med. 2001 Feb 19;193(4):497-507. doi: 10.1084/jem.193.4.497.
5
The DNA-binding domain in the Bacillus subtilis transition-state regulator AbrB employs significant motion for promiscuous DNA recognition.枯草芽孢杆菌过渡态调节因子AbrB中的DNA结合结构域利用显著的运动进行混杂性DNA识别。
J Mol Biol. 2001 Jan 19;305(3):429-39. doi: 10.1006/jmbi.2000.4305.
6
HYDRONMR: prediction of NMR relaxation of globular proteins from atomic-level structures and hydrodynamic calculations.HYDRONMR：基于原子水平结构和流体动力学计算预测球状蛋白质的核磁共振弛豫
J Magn Reson. 2000 Nov;147(1):138-46. doi: 10.1006/jmre.2000.2170.
7
HIV-2 and SIV nef proteins target different Src family SH3 domains than does HIV-1 Nef because of a triple amino acid substitution.由于存在一个三联氨基酸取代，与HIV-1 Nef相比，HIV-2和SIV的nef蛋白靶向不同的Src家族SH3结构域。
J Biol Chem. 2000 Feb 11;275(6):4171-6. doi: 10.1074/jbc.275.6.4171.
8
Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex.钙调蛋白-肽复合物形成时侧链熵的重新分布与损失。
Nat Struct Biol. 2000 Jan;7(1):72-7. doi: 10.1038/71280.
9
Characterization of TCR-induced receptor-proximal signaling events negatively regulated by the protein tyrosine phosphatase PEP.
Eur J Immunol. 1999 Dec;29(12):3845-54. doi: 10.1002/(SICI)1521-4141(199912)29:12<3845::AID-IMMU3845>3.0.CO;2-U.
10
Increased protein backbone conformational entropy upon hydrophobic ligand binding.疏水配体结合后蛋白质主链构象熵增加。
Nat Struct Biol. 1999 Dec;6(12):1118-21. doi: 10.1038/70057.
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