在神经母细胞瘤中，细胞分裂周期蛋白25B（cdc25B）信使核糖核酸（mRNA）的表达与N-原癌基因（N-myc）的表达相关。
Expression of the cdc25B mRNA correlated with that of N-myc in neuroblastoma.
作者信息
Sato Y, Sasaki H, Kondo S, Fukai I, Kiriyama M, Yamakawa Y, Fujii Y
机构信息
Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan.
出版信息
Jpn J Clin Oncol. 2001 Sep;31(9):428-31. doi: 10.1093/jjco/hye093.
BACKGROUND
Neuroblastoma is one of the most common solid tumors in early childhood. Overexpression of the proto-oncogene N-myc has been reported to be correlated with more malignant course of the disease. cdc25B is reported to be a target of myc and elevated in several malignant cells and tissues.
METHODS
Expression of cdc25B and N-myc messenger RNAs were evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) assay in 20 tumor samples from neuroblastoma using LightCycler. The data were analyzed with reference to clinicopathological factors. Immunohistochemistry for cdc25B was also performed.
RESULTS
There was no significant difference in the cdc25B expression between patient groups according to age, gender and clinical stage. The cdc25B mRNA expression levels were significantly correlated with N-myc mRNA levels (y = -0.445 + 20.577x, p < 0.0001).
CONCLUSION
We could not establish the clinical significance to determine the cdc25B mRNA level from neuroblastoma. However, we suggest that cdc25B may play an active role as a target of N-myc in neuroblastoma, although the biological function of cdc25B in neuroblastoma remains to be clarified.
背景
神经母细胞瘤是幼儿期最常见的实体瘤之一。据报道，原癌基因N - myc的过表达与该疾病更恶性的病程相关。据报道，细胞周期蛋白磷酸酶25B（cdc25B）是myc的一个靶点，在几种恶性细胞和组织中表达升高。
方法
使用LightCycler通过实时逆转录聚合酶链反应（RT - PCR）分析，对20例神经母细胞瘤肿瘤样本中的cdc25B和N - myc信使核糖核酸（mRNA）表达进行评估。参考临床病理因素对数据进行分析。还进行了cdc25B的免疫组化检测。
结果
根据年龄、性别和临床分期，患者组之间cdc25B表达无显著差异。cdc25B mRNA表达水平与N - myc mRNA水平显著相关（y = -0.445 + 20.577x，p < 0.0001）。
结论
我们无法确定神经母细胞瘤中cdc25B mRNA水平的临床意义。然而，我们认为cdc25B可能作为N - myc的一个靶点在神经母细胞瘤中发挥积极作用，尽管cdc25B在神经母细胞瘤中的生物学功能仍有待阐明。
Zotero 插件