人精子中各种CDC25B亚型的表达
Expression of various CDC25B isoforms in human spermatozoa.
作者信息
Teng Yen-Ni, Chung Chia-Ling, Lin Yung-Ming, Pan Hsien-An, Liao Rui-Wen, Kuo Pao-Lin
机构信息
Department of Biotechnology, Chia Nan University of Pharmacy and Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan.
出版信息
Fertil Steril. 2007 Aug;88(2):379-82. doi: 10.1016/j.fertnstert.2006.11.186. Epub 2007 Mar 6.
OBJECTIVE
To explore the role of CDC25B protein in postmeiotic germ cells.
DESIGN
In vitro experiment.
SETTING
University-based reproductive genetics laboratory.
PATIENT(S): Fertile and infertile volunteers.
INTERVENTION(S): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B.
MAIN OUTCOME MEASURE
Expression profiling of CDC25B in human spermatozoa.
RESULT(S): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia.
CONCLUSION(S): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
目的
探讨CDC25B蛋白在减数分裂后生殖细胞中的作用。
设计
体外实验。
单位
大学附属生殖遗传学实验室。
研究对象
有生育能力和无生育能力的志愿者。
干预措施
逆转录-聚合酶链反应（RT-PCR）、实时RT-PCR以及对CDC25B进行免疫染色。
主要观察指标
人精子中CDC25B的表达谱。
结果
人精子中表达四种剪接变体（CDC25B1、B2、B3和B4）。免疫荧光染色显示精子中段有强烈的均匀染色，而头部主段和细胞质中有较弱染色。弱精子症男性精子样本中CDC25B的信使核糖核酸（mRNA）转录水平升高。
结论
CDC25B在人精子不同细胞区室中的表达表明，CDC25B在终末分化的人生殖细胞中存在多种非细胞周期相关功能。
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