Milon B, Dhermy D, Pountney D, Bourgeois M, Beaumont C
INSERM U409 and IFR Claude Bernard, Faculté Xavier Bichat, P.O. Box 416, 16 Rue Henri Huchard, 75018 Cedex 18, Paris, France.
FEBS Lett. 2001 Nov 2;507(3):241-6. doi: 10.1016/s0014-5793(01)02950-7.
Two human divalent cation transporters of the ZIP family, hZip1 and hZip2, homologous to Irt1 (Arabidopsis thaliana), the first identified member, have been described. They were shown by transfection into K562 cells to be localized at the plasma membrane and to mediate zinc uptake. Here we report a differential subcellular localization of hZip1 according to cell type. By transient expressions of EGFP-hZip1, FLAG-tagged or native hZip1, we observed that hZip1 has a vesicular localization in COS-7 cells or in several epithelial cell lines, corresponding partially to the endoplasmic reticulum. Using anti-hZip1 antibodies, we confirmed the intracellular localization of the endogenous protein in PC-3, a prostate cancer cell line.
已发现人类ZIP家族的两种二价阳离子转运蛋白hZip1和hZip2，它们与首个被鉴定出的成员Irt1（拟南芥）同源。通过转染到K562细胞中显示，它们定位于质膜并介导锌摄取。在此，我们报道了hZip1根据细胞类型的不同亚细胞定位。通过瞬时表达EGFP-hZip1、FLAG标签化的或天然的hZip1，我们观察到hZip1在COS-7细胞或几种上皮细胞系中具有囊泡定位，部分对应于内质网。使用抗hZip1抗体，我们证实了内源性蛋白在前列腺癌细胞系PC-3中的细胞内定位。
