Pilger A, Germadnik D, Riedel K, Meger-Kossien I, Scherer G, Rüdiger H W
Department of Occupational Medicine, University of Vienna, A-1090 Vienna, Austria.
Free Radic Res. 2001 Sep;35(3):273-80. doi: 10.1080/10715760100300811.
Numerous studies have investigated the urinary excretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for the assessment of oxidative DNA damage in humans. In this study, we performed six consecutive series of measurement of urinary levels of 8-OHdG in 68 healthy probands, in order to provide information on the intra- and inter-individual variability of 8-OHdG and to estimate the influence of smoking, age, sex, body weight and body mass index (BMI) on the excretion of 8-OHdG. The intra-individual coefficient of variation (CV) of urinary 8-OHdG/24 h ranged from 0.18 to 1.06 (mean CV = 0.48). Women excreted significantly lower amounts of 8-OHdG/24 h than men, but the difference lost its significance when the body weight or urinary creatinine were used as covariates. By multiple linear regression analysis significant correlations between the mean individual levels of 8-OHdG/24 h excretion and urinary creatinine (rp = 0.61), and cotinine (rp = 0.27) have been observed, whereas no statistically significant effect of age, body weight and BMI was found. The 8-OHdG/creatinine ratio was found to be significantly increased in 23 smokers (1.95 +/- 0.40 mumol/mol) opposed to 45 non-smoking probands (1.62 +/- 0.50 mumol/mol), which is in good agreement with previously published data. No effect of passive smoking on the excretion of 8-OHdG was found. From our data we conclude that the intra-individual variability of urinary 8-OHdG excretion has been underestimated so far, indicating that values of 8-OHdG measured by single spot monitoring are not representative for individual base levels.
众多研究已将8-羟基-2'-脱氧鸟苷（8-OHdG）的尿排泄作为评估人类氧化DNA损伤的生物标志物进行了调查。在本研究中，我们对68名健康受试者连续进行了6组尿8-OHdG水平的测量，以便提供有关8-OHdG个体内和个体间变异性的信息，并估计吸烟、年龄、性别、体重和体重指数（BMI）对8-OHdG排泄的影响。尿8-OHdG/24小时的个体内变异系数（CV）范围为0.18至1.06（平均CV = 0.48）。女性24小时排泄的8-OHdG量显著低于男性，但当以体重或尿肌酐作为协变量时，这种差异失去了显著性。通过多元线性回归分析，观察到8-OHdG/24小时排泄的个体平均水平与尿肌酐（rp = 0.61）和可替宁（rp = 0.27）之间存在显著相关性，而未发现年龄、体重和BMI有统计学显著影响。与45名非吸烟受试者（1.62 +/- 0.50 μmol/mol）相比，23名吸烟者的8-OHdG/肌酐比值显著升高（1.95 +/- 0.40 μmol/mol），这与先前发表的数据高度一致。未发现被动吸烟对8-OHdG排泄有影响。根据我们的数据，我们得出结论，迄今为止尿8-OHdG排泄的个体内变异性被低估了，这表明通过单次即时监测测量的8-OHdG值不能代表个体碱基水平。
