[吸烟状况或年龄对电子电气设备拆解工人尿中8-羟基-2'-脱氧鸟苷的影响]

[Smoking status or age impact on urinary 8-hydroxy-2'-deoxyguanosine in electrical and electronic equipment waste dismantling workers].

作者信息

Wen Sheng, Gong Yan, Li Jing-Guang, Shi Ting-Ming, Zhao Yun-Feng, Xu Ying, Wu Yong-Ning

机构信息

National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Jun;43(6):474-7.

DOI:

PMID:19950712

Abstract

OBJECTIVE

To investigate the smoking or age impact on occupational workers in electrical and electronic equipment waste (e-waste) dismantling procedure, using 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in the urine as a biomarker for oxidative damage to DNA.

METHODS

The pre-workshift and post-workshift urinary samples of 64 male workers in e-waste dismantling procedure were detected by solid-phase extraction-high performance liquid chromatography-electrochemical detector (SPE-HPLC-ECD). The data were statistically analyzed by two factors, age or smoking status.

RESULTS

The 8-OHdG levels in non-smokers' urines (n = 42) were higher than those in smokers' urines (n = 22). The levels in pre-workshift urines were detected at (8.25 +/- 4.23) micromol/mol creatinine in non-smokers, while the values were (5.44 +/- 1.18) micromol/mol in smokers. And, the levels in post-workshift were detected at (43.12 +/- 16.19) micromol/mol creatinine in non-smokers, while the values were (14.82 +/- 2.51) micromol/mol in smokers. The 8-OHdG levels in pre-workshift urines were not different between non-smokers and smokers (t = -0.81, P = 0.42), however after 1 day exposure, urinary 8-OHdG levels were significantly increased in non-smokers than those in smokers (t = - 2.33, P < 0.05). On the other hand, the subjects were divided into five groups according to their age. The 8-OHdG levels in pre-workshift urines were (1.86 +/- 0.66), (3.57 +/- 0.54), (8.12 +/- 4.10), (11.39 +/- 3.70) micromol/mol creatinine in < 20 years group (n = 6), 20 -years group (n = 22), 30 - years group ( n = 23), 40 - 49 years group (n = 11) respectively. No effect of age was found on the pre-workshift urinary 8-OHdG levels (F = 0.98, t = 0.41). However, it was found that the post-workshift urinary 8-OHdG levels increased along with the e-waste workers' age (F = 4.81, P = 0.03), and they were (4.19 +/- 2.85), (19.89 +/- 5.26), (28.89 +/- 14.61), (34.94 +/- 12.50) micromol/mol creatinine in < 20 years group, 20 - years group, 30 - years group, 40 - 49 years group respectively.

CONCLUSION

The urinary 8-OHdG levels in the e-waste dismantling workers might be inhibited by smoking status. The post-workshift urinary 8-OHdG levels increased along with the e-waste workers' age.

摘要

目的

以尿中8-羟基-2'-脱氧鸟苷（8-OHdG）作为DNA氧化损伤的生物标志物，研究吸烟和年龄对电子电气设备废弃物（电子垃圾）拆解作业工人的影响。

方法

采用固相萃取-高效液相色谱-电化学检测器（SPE-HPLC-ECD）检测64名从事电子垃圾拆解作业男性工人班前和班后的尿样。数据按年龄或吸烟状况这两个因素进行统计学分析。

结果

非吸烟者（n = 42）尿中8-OHdG水平高于吸烟者（n = 22）。非吸烟者班前尿中8-OHdG水平为（8.25±4.23）微摩尔/摩尔肌酐，而吸烟者为（5.44±1.18）微摩尔/摩尔。非吸烟者班后尿中8-OHdG水平为（43.12±16.19）微摩尔/摩尔肌酐，吸烟者为（14.82±2.51）微摩尔/摩尔。非吸烟者和吸烟者班前尿中8-OHdG水平无差异（t = -0.81，P = 0.42），但暴露1天后，非吸烟者尿中8-OHdG水平显著高于吸烟者（t = -2.33，P < 0.05）。另一方面，根据年龄将受试者分为五组。<20岁组（n = 6）、20岁组（n = 22）、30岁组（n = 23）、40 - 49岁组（n = 11）班前尿中8-OHdG水平分别为（1.86±0.66）、（3.57±0.54）、（8.12±4.10）、（11.39±3.70）微摩尔/摩尔肌酐。年龄对班前尿中8-OHdG水平无影响（F = 0.98，t = 0.41）。然而，发现班后尿中8-OHdG水平随电子垃圾拆解工人年龄增加而升高（F = 4.81，P = 0.03），<20岁组、20岁组、30岁组、40 - 49岁组班后尿中8-OHdG水平分别为（4.19±2.85）、（19.89±5.26）、（28.89±14.61）、（34.94±12.50）微摩尔/摩尔肌酐。