Santus R, Patterson L K, Filipe P, Morlière P, Hug G L, Fernandes A, Mazière J C
Radiation Laboratory, University of Notre Dame, Notre Dame, In. 46556, USA.
Free Radic Res. 2001 Aug;35(2):129-36. doi: 10.1080/10715760100300671.
The kinetics of several processes involving the potential antioxidant role of urate in physiological systems have been investigated by pulse radiolysis. While the monoanionic urate radical, .UH-, can be produced directly by oxidation with .Br2- or .OH, it can also be generated by oxidation with the neutral tryptophan radical, .Trp, with a rate constant of 2 x 10(7) M-1s-1. This radical, .UH-, reacts with .O2- with a rate constant of 8 x 10(8) M-1s-1. Also, .UH- is reduced by flavonoids, quercetin and rutin in CTAB micelles at rate constants of 6 x 10(6) M-1s-1 and 1 x 10(6) M-1s-1, respectively. These results can be of value by providing reference data useful in further investigation of the antioxidant character of urate in more complex biological systems.
通过脉冲辐解研究了尿酸盐在生理系统中潜在抗氧化作用的几个过程的动力学。单阴离子尿酸盐自由基（.UH-）可通过与.Br2-或.OH氧化直接产生，也可由中性色氨酸自由基（.Trp）氧化产生，速率常数为2×10(7) M-1s-1。该自由基（.UH-）与.O2-反应的速率常数为8×10(8) M-1s-1。此外，在CTAB胶束中，.UH-分别被类黄酮、槲皮素和芦丁以6×10(6) M-1s-1和1×10(6) M-1s-1的速率常数还原。这些结果通过提供有用的参考数据，对进一步研究尿酸盐在更复杂生物系统中的抗氧化特性具有重要价值。
