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J Bacteriol. 2001 Dec;183(23):6947-50. doi: 10.1128/JB.183.23.6947-6950.2001.
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Doc-mediated cell killing in Shigella flexneri using a C1/LacI controlled expression system.使用C1/LacI控制表达系统在福氏志贺菌中进行Doc介导的细胞杀伤。
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Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.用于致病性革兰氏阳性菌的热调节广谱启动子系统的开发。
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Use of genetically engineered phage to deliver antimicrobial agents to bacteria: an alternative therapy for treatment of bacterial infections.利用基因工程噬菌体向细菌递送抗菌剂：一种治疗细菌感染的替代疗法。
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Construction and characterization of regulated L-arabinose-inducible broad host range expression vectors in Xanthomonas.黄单胞菌中受调控的L-阿拉伯糖诱导型广宿主范围表达载体的构建与表征
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Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum.大肠杆菌araBAD启动子在谷氨酸棒杆菌中的正调控表达
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Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.携带L-阿拉伯糖诱导型大肠杆菌araBAD启动子和araC调节因子的广宿主范围表达载体。
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Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors.克雷伯菌属作为医院病原体：流行病学、分类学、分型方法及致病因素
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Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.通过LacR/O、TetR/O和AraC/I1-I2调控元件对大肠杆菌转录单元进行独立且严格的调控。
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The tripartite immunity system of phages P1 and P7.噬菌体P1和P7的三方免疫系统。
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利用噬菌体P1衍生的C1调控启动子系统在肺炎克雷伯菌和弗氏志贺菌中进行可控表达。

Controlled expression in Klebsiella pneumoniae and Shigella flexneri using a bacteriophage P1-derived C1-regulated promoter system.

作者信息

Schofield D A, Westwater C, Dolan J W, Schmidt M G, Norris J S

机构信息

Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave., Charleston, SC 29403, USA.

出版信息

J Bacteriol. 2001 Dec;183(23):6947-50. doi: 10.1128/JB.183.23.6947-6950.2001.

DOI:10.1128/JB.183.23.6947-6950.2001
PMID:11698385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC95537/
Abstract

The utility of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor was examined in Shigella flexneri and Klebsiella pneumoniae. Promoters carrying C1 operator sites driving LacZ expression had induction/repression ratios of up to 240-fold in S. flexneri and up to 50-fold in K. pneumoniae. The promoters exhibited remarkably low basal expression, demonstrated modulation by temperature, and showed rapid induction. This system will provide a new opportunity for controlled gene expression in enteric gram-negative bacteria.

摘要

在福氏志贺菌和肺炎克雷伯菌中检测了由噬菌体P1温度敏感型C1阻遏物调控的启动子的效用。携带驱动LacZ表达的C1操纵位点的启动子在福氏志贺菌中的诱导/抑制比高达240倍，在肺炎克雷伯菌中高达50倍。这些启动子表现出极低的基础表达，受温度调节，并显示出快速诱导。该系统将为肠道革兰氏阴性菌中可控基因表达提供新的机会。