利用噬菌体P1衍生的C1调控启动子系统在肺炎克雷伯菌和弗氏志贺菌中进行可控表达。
Controlled expression in Klebsiella pneumoniae and Shigella flexneri using a bacteriophage P1-derived C1-regulated promoter system.
作者信息
Schofield D A, Westwater C, Dolan J W, Schmidt M G, Norris J S
机构信息
Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave., Charleston, SC 29403, USA.
出版信息
J Bacteriol. 2001 Dec;183(23):6947-50. doi: 10.1128/JB.183.23.6947-6950.2001.
Abstract
The utility of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor was examined in Shigella flexneri and Klebsiella pneumoniae. Promoters carrying C1 operator sites driving LacZ expression had induction/repression ratios of up to 240-fold in S. flexneri and up to 50-fold in K. pneumoniae. The promoters exhibited remarkably low basal expression, demonstrated modulation by temperature, and showed rapid induction. This system will provide a new opportunity for controlled gene expression in enteric gram-negative bacteria.
摘要
在福氏志贺菌和肺炎克雷伯菌中检测了由噬菌体P1温度敏感型C1阻遏物调控的启动子的效用。携带驱动LacZ表达的C1操纵位点的启动子在福氏志贺菌中的诱导/抑制比高达240倍，在肺炎克雷伯菌中高达50倍。这些启动子表现出极低的基础表达，受温度调节，并显示出快速诱导。该系统将为肠道革兰氏阴性菌中可控基因表达提供新的机会。
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