White S L, Bertrand J A, Wade M R, Washburn S P, Green J T, Jenkins T C
Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
J Dairy Sci. 2001 Oct;84(10):2295-301. doi: 10.3168/jds.S0022-0302(01)74676-0.
Holstein (n = 19) and Jersey (n = 18) cows were used to study effects of two feeding systems on fatty acid composition of milk. Confinement cows were fed a total mixed ration with corn silage and alfalfa silage and pastured cows grazed a crabgrass (90%) and clover (10%) pasture and were allowed 5.5 kg of grain per head daily. Two milk samples were collected from each cow at morning and afternoon milkings 1 d each week for four consecutive weeks in June and July 1998. One set of milk samples was analyzed to determine fatty acid composition, and the second set was used for crude protein and total fat analyses. Data were analyzed by the general linear models procedure of SAS, using a split-plot model with breed, treatment, and breed x treatment as main effects and time of sampling and week as subplot effects along with appropriate interactions. Milk from pastured cows was higher than milk from confinement cows for the cis-9, trans-11 octadecadienoic acid isomer of conjugated linoleic acid (CLA). Also, milk from Holsteins was higher than milk from Jerseys for C16:1, C18:1, and CLA and lower than Jerseys for C6:0, C8:0, C10:0, C12:0, and C14:0. Several treatment x week interactions existed, but main effects were still important; for example, proportions of CLA in milk of grazed cows were relatively constant across weeks (0.66, 0.64, 0.64, and 0.69% +/- 0.02%, respectively), but the CLA in milk of confinement cows increased in wk 4 (0.35, 0.31, 0.31, and 0.48% +/- 0.02% for wk 1 to 4, respectively). There are potentially important differences in fatty acid composition of milk from cows consuming a warm season pasture species compared with milk from cows consuming a total mixed ration, as well as differences between Holstein and Jersey breeds.
选用荷斯坦奶牛(n = 19头)和泽西奶牛(n = 18头)研究两种饲养系统对牛奶脂肪酸组成的影响。舍饲奶牛饲喂由玉米青贮和苜蓿青贮组成的全混合日粮,放牧奶牛在狗尾草(90%)和三叶草(10%)的牧场上放牧,且每头奶牛每天补饲5.5千克谷物。1998年6月和7月,连续四周每周从每头奶牛的早晚两次挤奶中各采集两份牛奶样本。一组牛奶样本用于分析脂肪酸组成,另一组用于粗蛋白和总脂肪分析。数据采用SAS的通用线性模型程序进行分析,使用裂区模型,以品种、处理方式以及品种×处理方式作为主效应,采样时间和周作为副区效应以及适当的交互作用。放牧奶牛所产牛奶中,共轭亚油酸(CLA)的顺-9,反-11十八碳二烯酸异构体含量高于舍饲奶牛所产牛奶。此外,荷斯坦奶牛所产牛奶中C16:1、C18:1和CLA的含量高于泽西奶牛所产牛奶,而C6:0、C8:0、C10:0、C12:0和C14:0的含量低于泽西奶牛所产牛奶。存在几个处理方式×周的交互作用,但主效应仍然很重要;例如,放牧奶牛所产牛奶中CLA的比例在各周相对稳定(分别为0.66%、0.64%、0.64%和0.69%±0.02%),但舍饲奶牛所产牛奶中的CLA在第4周有所增加(第1至4周分别为0.35%、0.31%、0.31%和0.48%±0.02%)。与采食全混合日粮的奶牛所产牛奶相比,采食暖季牧草品种的奶牛所产牛奶在脂肪酸组成上可能存在重要差异,荷斯坦奶牛和泽西奶牛品种之间也存在差异。