Huang R K, Wang C H
National Insititute of Environmental Analysis, Government of the Republic of China, Chungli, Taiwan.
Proc Natl Sci Counc Repub China B. 2001 Oct;25(4):248-52.
Nonylphenol and octylphenol, the major biodegradation products of nonionic surfactants, are among the increasing number of widely used industrial chemicals that are recognized as endocrine disrupters. In this study, nonylphenol and octylphenol were administered to male carp by injection, feeding and immersion, and a validated enzyme-linked immunosorbent assay (ELISA) was used to monitor the plasma levels of the yolk precursor vitellogenin over time. In injection and feeding experiments, vitellogenin levels increased significantly after 14 days. After immersion for 14 and 28 days, respectively, significantly higher concentrations of plasma vitellogenin were detected in male carp exposed to 4 microg/L nonylphenol and octylphenol. When transferred to clean water, the elevated plasma vitellogenin levels in carp exposed to 4 microg/L octylphenol for 42 days returned to control levels within 28 days.
壬基酚和辛基酚是非离子表面活性剂的主要生物降解产物，属于越来越多被确认为内分泌干扰物的广泛使用的工业化学品。在本研究中，通过注射、投喂和浸泡的方式给雄性鲤鱼施用壬基酚和辛基酚，并使用经过验证的酶联免疫吸附测定（ELISA）来监测血浆中卵黄前体卵黄蛋白原的水平随时间的变化。在注射和投喂实验中，14天后卵黄蛋白原水平显著升高。在分别浸泡14天和28天后，暴露于4微克/升壬基酚和辛基酚的雄性鲤鱼血浆中检测到显著更高浓度的卵黄蛋白原。当转移到清洁水中时，暴露于4微克/升辛基酚42天的鲤鱼血浆中升高的卵黄蛋白原水平在28天内恢复到对照水平。
