Sukwit S, Chuenchitra T, Samakoses R, Songprasom K, Aree C, Akapirat S, Panjapornsuk K, Ubol S, Nitayaphan S
Department of Microbiology, Thai Component, Armed Forces Research Institute of Medical Sciences, Bangkok.
Asian Pac J Allergy Immunol. 2001 Jun;19(2):107-13.
The objective of this study was to determine changes in Th1/Th2 cytokine production at the cellular level which occur during the progression of HIV-1 subtype E infection in Thai children born to HIV-1 subtype E infected mothers. Mitogen stimulated whole blood cultures from 12 uninfected and 27 HIV-1 subtype E infected Thai children were stained intracellularly with fluorescein labelled monoclonal antibodies against Interleukin (IL)-2 and IFN-gamma (Th1 cytokines) and IL-4 (Th2 cytokine). Additionally, co-staining of CD4+ and CD8+ T cells was performed. Results were analyzed by two and three color flow cytometry. The percentage of IFN-gamma expressing cells in CD4+ T cells was increased in HIV-1 subtype E infected Thai children with mild and moderate immunosuppression (Immunological categories 1 + 2, Centers for Diseases Control and Prevention (CDC) staging system, 1994). The percentages of IFN-gamma expression was continuously enhanced accompanied by remaining preserved in the proportion of IL-2 producing T cells in HIV-1 subtype E infected Thai children with severe immunosuppression (Immunological category 3, CDC staging system, 1994). The percentages of IFN-gamma expression was continuously augmented whereas the proportion of IL-2 producing T cells remained unchanged in HIV-1 subtype E infected Thai Children with severe immunosuppression (immunological category 3, CDC staging system, 1994). The percentage of Th2 cytokine producing cells within the CD4+ ad CD8+ T cells increased in HIV-1 subtype E infected individuals and showed a significant difference in HIV-1 subtype E infected Thai children with AIDS compared with uninfected infants. These results suggest that in vertically acquired HIV-1 infection with severe immunosuppression, the percentages of IL-2 producing CD4+ T cell was consistent but the percentages of IL-4 and IFN-gamma producing cell were increased. Similar results were found for CD8+ T cells in which IL-4 producing cells were increased in conjunction with a remaining in the number of IL-2 producing cells in HIV-1 subtype E infected Thai children. Thus, changes in the Th1 and Th2 cytokine pattern during HIV-1 infection may contribute to the prognosis of HIV disease in children.
本研究的目的是确定在感染HIV-1 E亚型的泰国母亲所生儿童中，HIV-1 E亚型感染进展过程中细胞水平上Th1/Th2细胞因子产生的变化。对12名未感染和27名感染HIV-1 E亚型的泰国儿童的丝裂原刺激全血培养物进行细胞内染色，使用针对白细胞介素（IL）-2、干扰素-γ（Th1细胞因子）和IL-4（Th2细胞因子）的荧光素标记单克隆抗体。此外，还对CD4+和CD8+ T细胞进行了共染色。通过双色和三色流式细胞术分析结果。在轻度和中度免疫抑制的感染HIV-1 E亚型的泰国儿童中（免疫分类1 + 2，疾病控制和预防中心（CDC）分期系统，1994年），CD4+ T细胞中表达干扰素-γ的细胞百分比增加。在严重免疫抑制的感染HIV-1 E亚型的泰国儿童中（免疫分类3，CDC分期系统，1994年），干扰素-γ表达百分比持续增强，同时产生IL-2的T细胞比例保持不变。在严重免疫抑制的感染HIV-1 E亚型的泰国儿童中（免疫分类3，CDC分期系统，1994年），干扰素-γ表达百分比持续增加，而产生IL-2的T细胞比例保持不变。在感染HIV-1 E亚型的个体中，CD4+和CD8+ T细胞内产生Th2细胞因子的细胞百分比增加，与未感染婴儿相比，感染HIV-1 E亚型的艾滋病泰国儿童存在显著差异。这些结果表明，在垂直感染HIV-1且伴有严重免疫抑制的情况下，产生IL-2的CD4+ T细胞百分比保持一致，但产生IL-4和干扰素-γ的细胞百分比增加。在感染HIV-1 E亚型的泰国儿童的CD8+ T细胞中也发现了类似结果，其中产生IL-4的细胞增加，同时产生IL-2的细胞数量保持不变。因此，HIV-1感染期间Th1和Th2细胞因子模式的变化可能有助于儿童HIV疾病的预后。
