Azizah M R, Ainoi S S, Kuak S H, Kong N C, Normaznah Y, Rahim M N
Biotechnology Centre, Institute for Medical Research, Kuala Lumpur, Malaysia.
Asian Pac J Allergy Immunol. 2001 Jun;19(2):93-100.
The frequency of the HLA class II antigens/alleles (HLA-DR, DQ and DP) were studied in 70 Malaysian Chinese patients with systemic lupus erythematosus (SLE) to examine the contribution of these genes to disease susceptibility, their clinical expression and Immunological responses. This was done using modified PCR-RFLP technique. These samples were then compared with 66 ethnically matched controls. We found a strong association of the DQA10102 (p corr = 0.032, rr = 3.39), DQB10501 (p corr = 0.003, rr = 4.55), 0601 (p corr = 0.006, rr = 4.22) and DPB1 0901(p corr = 0.02, rr = 4.58) with SLE. Clinically, we found a strong association of DR2 and DQA10301 with renal involvement and DQA10102 with alopecia. Immunologically, statistical analysis (Chi-square test ) showed a strong association of DQA10102 with anti-Ro/La antibodies while DQA10301 was observed to be strongly associated with antibodies to ds DNA. DQA1*0102 was found more frequently in those with a later disease onset (30 years of age or above). From these data we suggest that the HLA class II genes play a role in conferring disease susceptibility and clinical and immunological expression.
我们研究了70名马来西亚华裔系统性红斑狼疮（SLE）患者的HLA II类抗原/等位基因（HLA - DR、DQ和DP）的频率，以检验这些基因对疾病易感性、临床表型及免疫反应的影响。采用改良的聚合酶链反应-限制性片段长度多态性（PCR - RFLP）技术进行检测。随后将这些样本与66名种族匹配的对照进行比较。我们发现DQA10102（校正p值=0.032，相对危险度rr = 3.39）、DQB10501（校正p值=0.003，rr = 4.55）、0601（校正p值=0.006，rr = 4.22）和DPB10901（校正p值=0.02，rr = 4.58）与SLE显著相关。临床上，我们发现DR2和DQA1*
0301与肾脏受累显著相关，而DQA10102与脱发显著相关。免疫方面，统计学分析（卡方检验）显示DQA10102与抗Ro/La抗体显著相关，而DQA10301与抗双链DNA抗体显著相关。DQA10102在发病较晚（30岁及以上）的患者中更为常见。基于这些数据，我们认为HLA II类基因在赋予疾病易感性以及临床和免疫表型方面发挥作用。
