成年大鼠肾脏各区域对前列腺素生物合成的一种分离纯化中间产物的分解代谢作用。

Catabolism of an isolated, purified intermediate of prostaglandin biosynthesis by regions of the adult rat kidney.

作者信息

Pace-Asciak C, Nashat M

出版信息

Biochim Biophys Acta. 1975 May 22;388(2):243-52. doi: 10.1016/0005-2760(75)90129-0.

DOI:10.1016/0005-2760(75)90129-0

PMID:1169976

Abstract

A heat labile, cold-stable, stannous chloride-reducible intermediate of prostaglandin biosynthesis was formed in good yield (greater than 60%) from 3H-labeled arachidonic acid during brief incubations (30--90 s, 37 degrees C) with sheep seminal vesicle microsomes in the presence of p-hydroxymercuribenzoate (4 mM). This intermediate appears to have properties similar to one of the endoperoxides (15-hydroxyprostaglandin-9,11-endoperoxide) recently isolated by Hamberg and Samuelsson (Proc. Natl. Acad. Sci. U.S. (1973) 70, 889-903) AND Nugteren and Hazelhof (Biochem. Biophys. Acta. (1973) 326, 448-461). 2. Treatment of the purified intermediate with homogenates of rat kidney cortex, medulla and papilla resulted within 2 min (37 degrees C) in complete conversion into several compounds including prostaglandins E2 and F2alpha. The main product (40-50% yield formed by papilla homogenates was prostaglandin E2. The conversion into prostaglandin E2 was largely abolished by previous bo9ling of the homogenate whereas the conversion into prostaglandin F2alpha was not. The intermediate was stable in buffer for the same period of incubation. 3. The ratio of tritiated prostaglandins E2: F2alpha obtained were: papilla, 1.90; medulla, 0.76; cortex, 0.48. 4. These observations indicate that both types of prostaglandins can be formed by all three regions of the rat kidney and that regional differences exist in the proportion of E2 : F2alpha that is formed. Whereas prostaglandin E2 is mostly formed by an enzymatic process, prostaglandin F2alpha is not.

摘要

在37℃下，将3H标记的花生四烯酸与含有4 mM对羟基汞苯甲酸的绵羊精囊微粒体短暂孵育（30 - 90秒），可高产率（大于60%）形成一种热不稳定、冷稳定、可被氯化亚锡还原的前列腺素生物合成中间体。该中间体的性质似乎与Hamberg和Samuelsson（《美国国家科学院院刊》（1973年）70卷，889 - 903页）以及Nugteren和Hazelhof（《生物化学与生物物理学学报》（1973年）326卷，448 - 461页）最近分离出的一种内过氧化物（15 - 羟基前列腺素 - 9,11 - 内过氧化物）相似。2. 用大鼠肾皮质、髓质和乳头的匀浆处理纯化后的中间体，在2分钟内（37℃）可完全转化为几种化合物，包括前列腺素E2和F2α。主要产物（乳头匀浆形成的产率为40 - 50%）是前列腺素E2。匀浆预先煮沸后，向前列腺素E2的转化基本被消除，而向前列腺素F2α的转化则不受影响。中间体在相同孵育时间内在缓冲液中稳定。3. 得到的氚标记前列腺素E2与F2α的比例为：乳头，1.90；髓质，0.76；皮质，0.48。4. 这些观察结果表明，大鼠肾脏的所有三个区域都能形成这两种类型的前列腺素，并且在形成的E2与F2α比例上存在区域差异。前列腺素E2大多通过酶促过程形成，而前列腺素F2α则不然。