Wlodawer P, Kindahl H, Hamberg M
Biochim Biophys Acta. 1976 Jun 22;431(3):603-14. doi: 10.1016/0005-2760(76)90224-1.
Formation of prostaglandin F2Alpha in the cow and guinea pig uterus microsomes was studied using 14C-labeled arachidonic acid and prostaglandin H2. The total conversion of arachidonic acid was of a low order and underwent fluctuations during the estrous cycle of the guinea pig, being highest towards the end of the cycle. Injections of beta-estradiol-3-benzoate also resulted in higher activity of the uterine prostaglandin synthetase. The uterine prostaglandin synthesizing system appeared to differ in several respects from that present in seminal vesicles, with regard to the proportions of the products formed and the effects of various agents, e.g. reduced glutathione. An inhibiting factor which supressed the fatty acid cyclo-oxygenase was found to be present in uterine preparations. Prostaglandin endoperoxide (prostaglandin H2) was very efficiently reduced to prostaglandin F2alpha by cow and guinea-pig uterus microsomes. Prostaglandin G2 also gave rise to prostaglandin F2alpha. Prostaglandin E2, on the other hand, was not reduced. Both the inhibiting factor and the endoperoxide reducing activity are likely to be parts of a highly specialized mechanism that modulates prostaglandin F2alpha formation in the uterus.
利用14C标记的花生四烯酸和前列腺素H2，对奶牛和豚鼠子宫微粒体中前列腺素F2α的形成进行了研究。花生四烯酸的总转化率较低，且在豚鼠发情周期中出现波动，在周期末期最高。注射β-雌二醇-3-苯甲酸酯也会导致子宫前列腺素合成酶的活性升高。子宫前列腺素合成系统在几个方面似乎与精囊中存在的系统不同，在产物形成比例和各种试剂（如还原型谷胱甘肽）的作用方面。发现子宫制剂中存在一种抑制脂肪酸环氧化酶的抑制因子。前列腺素内过氧化物（前列腺素H2）被奶牛和豚鼠子宫微粒体非常有效地还原为前列腺素F2α。前列腺素G2也能生成前列腺素F2α。另一方面，前列腺素E2不会被还原。抑制因子和内过氧化物还原活性可能都是调节子宫中前列腺素F2α形成的高度专业化机制的一部分。
