Aelion C M, Mathur P P
Department of Environmental Health Sciences, University of South Carolina, Columbia 29208, USA.
Environ Toxicol Chem. 2001 Nov;20(11):2411-9. doi: 10.1897/1551-5028(2001)020<2411:abtdad>2.0.co;2.
Atrazine, a triazine herbicide widely used in the United States, contributes to surface-water and groundwater contamination, as can deisopropylatrazine (DIA) and deethylatrazine (DEA), two of its microbial degradation products. Production of DIA and DEA by native bacteria in aquatic sediments has not been investigated thoroughly. We assessed atrazine and production of DIA and DEA over time in coastal aquatic sediments associated with different land uses including creeks from an undeveloped preserve and a suburban development, a golf course drainage ditch, and a contaminated commercial harbor. Sediments were incubated in microcosms, spiked with U-14C-atrazine, extracted, and analyzed for 14C in a liquid scintillation counter. Atrazine, DIA, and DEA also were quantified by gas chromatography-mass spectrometry. The amount of 14C recovered varied at each site as a function of the sediment organic carbon content and decreased significantly over time. Both DEA and DIA were measured primarily in the aqueous phase. Transformation was more extensive to DIA than to DEA. The ratio of DIA to atrazine recovered from the undeveloped preserve was as high as 0.13. In contrast, the golf course had limited biotransformation, and had the greatest atrazine recoveries so atrazine, not DEA and DIA, may have a greater impact at this site.
阿特拉津是一种在美国广泛使用的三嗪类除草剂，它会导致地表水和地下水污染，其两种微生物降解产物去异丙基阿特拉津（DIA）和去乙基阿特拉津（DEA）也会造成污染。水生沉积物中天然细菌产生DIA和DEA的情况尚未得到充分研究。我们评估了与不同土地利用相关的沿海水生沉积物中阿特拉津以及DIA和DEA随时间的产生情况，这些沉积物来自未开发保护区和郊区开发区的小溪、高尔夫球场排水沟以及受污染的商业港口。将沉积物置于微观环境中，加入U-14C-阿特拉津，进行提取，并在液体闪烁计数器中分析14C。阿特拉津、DIA和DEA也通过气相色谱-质谱法进行定量。每个地点回收的14C量因沉积物有机碳含量而异，并随时间显著减少。DEA和DIA主要在水相中检测到。向DIA的转化比向DEA的转化更广泛。从未开发保护区回收的DIA与阿特拉津的比率高达0.13。相比之下，高尔夫球场的生物转化有限，阿特拉津的回收率最高，因此在该地点，可能是阿特拉津而非DEA和DIA产生更大影响。
