Muyanga J, Matsuzaki Y, Sugawara K, Kimura K, Mizuta K, Ndumba I, Muraki Y, Tsuchiya E, Hongo S, Kasolo F C, Numazaki Y, Nakamura K
Department of Microbiology and Pathology, University Teaching Hospital, Lusaka, Zambia.
Arch Virol. 2001;146(9):1667-79. doi: 10.1007/s007050170055.
Previous studies of the hemagglutinin (HA) genes of various influenza B virus isolates demonstrated the existence of two antigenically distinct virus lineages represented by B/Victoria/2/87 and B/Yamagata/16/88, respectively. Here, we investigated the antigenic and genetic characteristics of influenza B viruses isolated from children living in Lusaka, Zambia between January and May 1999. Antigenic analysis with chicken antiviral sera showed that all the Zambian isolates had the HA protein belonging to B/Yamagata/16/88-related lineage. Furthermore, phylogenetic analyses of the eight RNA segments performed by using the total or partial nucleotide sequences of the two representative Zambian strains (B/Lusaka/270/99 and B/Lusaka/432/99) as well as the previously reported sequences suggested that the Zambian viruses are closely related to the recently circulating reassortants represented by B/Shiga/T30/98 and B/Yamanashi/166/98 which acquired the genes coding for three polymerase proteins (PB2, PB1, and PA), HA, nucleoprotein, and matrix protein from a B/Yamagata/16/88-like parent and the gene encoding nonstructural proteins (NS1 and NS2) from a B/Guandong/8/93-like parent.
以往对多种乙型流感病毒分离株血凝素（HA）基因的研究表明，存在两种抗原性不同的病毒谱系，分别以B/维多利亚/2/87和B/山形/16/88为代表。在此，我们调查了1999年1月至5月间从赞比亚卢萨卡儿童中分离出的乙型流感病毒的抗原性和基因特征。用鸡抗病毒血清进行的抗原分析表明，所有赞比亚分离株的HA蛋白都属于B/山形/16/88相关谱系。此外，通过使用两个代表性赞比亚毒株（B/卢萨卡/270/99和B/卢萨卡/432/99）的全部或部分核苷酸序列以及先前报道的序列对八个RNA片段进行系统发育分析表明，赞比亚病毒与最近流行的重配株密切相关，这些重配株以B/滋贺/T30/98和B/山梨/166/98为代表，它们从一个类似B/山形/16/88的亲本获得了编码三种聚合酶蛋白（PB2、PB1和PA）、HA、核蛋白和基质蛋白的基因，并从一个类似B/广东/8/93的亲本获得了编码非结构蛋白（NS1和NS2）的基因。
