Nair J, Gibbs R, Mathew K, Ho G E
Institute for Environmental Sciences, Murdoch University, Murdoch, Western Australia.
Water Sci Technol. 2001;44(6):119-26.
Rainwater, borewater and catchment water are used for domestic water supply purposes with or without treatment in remote areas around the world. These places seldom have any facilities for routine testing of their drinking water. A simple on-site testing method is highly required in such areas. The H2S method has been tested for treated drinking water and was found to have a good correlation with the standard methods. The present study was aimed at assessing the suitability of the H2S method for testing different sources of drinking water. Since these types of water may contain H2S producing bacteria not of faecal origin the occurrence of false results in this method cannot be overruled. Therefore it was worthwhile to study whether the positive results are true positive results and what percentage of false positive and false negative results could be expected while using this test for routine analysis of water samples. Results were compared with the results using standard procedures for testing total coliforms, Escherichia coli and Salmonella spp. The present experiment analysed 121 rainwater samples, 17 borewater samples, 41 catchment water samples and 74 remote Aboriginal community water samples. Rainwater, borewater and catchment water samples gave true results of 78.5%, 82.3% and 80.5% respectively while the treated and untreated community samples gave true results of 93.7 and 84.6% respectively. It was concluded that in the developing countries where the acceptable level of total coliform is <10 MPN, the H2S method would be a good test to identify microbial contamination. In other regions, the H2S method could be used as a screening test for drinking water supplies.
在世界偏远地区,雨水、井水和集水区水被用于家庭供水,有的经过处理,有的未经处理。这些地方很少有对饮用水进行常规检测的设施。在这些地区,非常需要一种简单的现场检测方法。硫化氢(H2S)方法已针对处理后的饮用水进行了测试,发现与标准方法有良好的相关性。本研究旨在评估H2S方法对不同饮用水源检测的适用性。由于这些类型的水中可能含有非粪便来源的产H2S细菌,因此不能排除该方法出现错误结果的可能性。因此,研究使用该测试对水样进行常规分析时,阳性结果是否为真阳性结果以及假阳性和假阴性结果的预期比例是很有必要的。将结果与使用检测总大肠菌群、大肠杆菌和沙门氏菌属的标准程序得到的结果进行比较。本实验分析了121个雨水样本、17个井水样本、41个集水区水样本和74个偏远原住民社区水样。雨水、井水和集水区水样的真阳性结果分别为78.5%、82.3%和80.5%,而处理过和未处理的社区水样的真阳性结果分别为93.7%和84.6%。得出的结论是,在总大肠菌群可接受水平<10 MPN的发展中国家,H2S方法将是识别微生物污染的良好测试方法。在其他地区,H2S方法可作为饮用水供应的筛查测试方法。