Del Bigio M R, Yan H J, Xue M
Department of Pathology, University of Manitoba, D212-770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3.
J Neurol Sci. 2001 Nov 15;192(1-2):53-9. doi: 10.1016/s0022-510x(01)00605-0.
Neuronal and glial cell death in the striatum of a rat model of collagenase-induced intracerebral hemorrhage begins at 1 day and continues for at least 3 weeks. We hypothesized that administration of a neurotrophic agent would reduce neuronal loss in this experimental model. Because it has been shown to protect striatal neurons against excitotoxic injury, a second-generation ciliary neurotrophic factor (CNTF) (AXOKINE) was administered by continuous intracerebral infusion (2 microg/day) beginning 28 h after hemorrhage and continuing for 2 weeks. Magnetic resonance imaging showed that the hematoma size was comparable in control and treated rats prior to treatment. Counts of medium-sized striatal neurons within 320 microm of the hematoma 8 weeks after the hemorrhage revealed a slight but statistically significant benefit with a 42.5% loss in treated rats compared to 51.7% loss in controls. The results suggest that AXOKINE might be protective of striatal neurons in the vicinity of a hemorrhagic lesion.
胶原酶诱导的大鼠脑出血模型纹状体中的神经元和神经胶质细胞死亡始于第1天，并持续至少3周。我们推测给予神经营养剂会减少该实验模型中的神经元损失。由于已证明第二代睫状神经营养因子（CNTF）（Axokine）可保护纹状体神经元免受兴奋性毒性损伤，因此在出血后28小时开始通过脑内持续输注（2微克/天）给予第二代睫状神经营养因子（Axokine），并持续2周。磁共振成像显示，治疗前对照组和治疗组大鼠的血肿大小相当。出血8周后，对血肿周围320微米范围内的中型纹状体神经元进行计数，结果显示有轻微但具有统计学意义的益处，治疗组大鼠的神经元损失率为42.5%，而对照组为51.7%。结果表明，Axokine可能对出血性病变附近的纹状体神经元具有保护作用。
