Burr Thomas J., Otten Leon
Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456.
Annu Rev Phytopathol. 1999;37:53-80. doi: 10.1146/annurev.phyto.37.1.53.
Not until 1973 was it reported that strains of Agrobacterium that cause crown gall disease of grape form a specific group (later characterized as Agrobacterium vitis). Tumorigenic and nontumorigenic A. vitis have since been isolated from infected and symptomless grapes worldwide. Research on the genetic makeup of A. vitis has led to an improved understanding of pathogen biology and bacterial evolution. In addition, the identification of significant gene sequences has facilitated the development of PCR and RFLP-based identification procedures that continue to improve the detection of A. vitis in plants and soil. Current control practices rely on the use of disease-resistant cultivars, cultural practices that minimize plant injury, and the production of pathogen-free vines. Promising future controls include employment of biological control agents and development of crown gall-resistant transgenic grapevines.
直到1973年,才有报道称引起葡萄冠瘿病的土壤杆菌菌株形成了一个特定的群体(后来被鉴定为葡萄土壤杆菌)。此后,在世界各地受感染和无症状的葡萄中分离出了致瘤性和非致瘤性的葡萄土壤杆菌。对葡萄土壤杆菌基因组成的研究有助于增进对病原体生物学和细菌进化的理解。此外,重要基因序列的鉴定促进了基于PCR和RFLP的鉴定方法的发展,这些方法不断改进对植物和土壤中葡萄土壤杆菌的检测。目前的防治措施依赖于使用抗病品种、尽量减少植物损伤的栽培措施以及生产无病原体的葡萄藤。未来有前景的防治方法包括使用生物防治剂和培育抗冠瘿病的转基因葡萄藤。