d'Estaing S G, Lornage J, Hadj S, Boulieu D, Salle B, Guérin J F
Département de Médecine de la Reproduction, Centre hospitalier de l'Université de Biologie de la reproduction, Hôpital Edouard Herriot, Lyon, France.
Fertil Steril. 2001 Nov;76(5):1032-5. doi: 10.1016/s0015-0282(01)02737-6.
To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period.
Retrospective study.
Institutional practice assisted reproductive technology center.
PATIENT(S): Ninety-nine selected patients undergoing IVF with blastocyst culture.
INTERVENTION(S): In vitro fertilization and transfer of at least one expanded blastocyst after culture using either Vero cells or sequential media (S(2) medium).
MAIN OUTCOME MEASURE(S): Blastocyst, pregnancy, and implantation rates.
RESULT(S): The blastocyst formation rate (41.6% vs. 31.6%) is significantly increased with sequential media compared with the coculture system, but there is no statistically significant difference in the pregnancy and implantation rates (39.5% vs. 38.6% and 23.7% vs. 15.1%, respectively).
CONCLUSION(S): Blastocyst culture on sequential media offers a better dynamic of embryo development (22% vs. 13.6% blastocyst at day 5). There was no statistically significant difference between the two groups in the take-home baby rate (18 vs. 12).
比较两种囊胚培养系统：同期在Vero细胞上培养和使用序贯培养基培养。
回顾性研究。
机构辅助生殖技术中心。
99例接受体外受精并进行囊胚培养的选定患者。
使用Vero细胞或序贯培养基（S(2)培养基）培养后进行体外受精并移植至少一枚扩张囊胚。
囊胚、妊娠及着床率。
与共培养系统相比，序贯培养基培养的囊胚形成率（41.6%对31.6%）显著提高，但妊娠率和着床率无统计学显著差异（分别为39.5%对38.6%和23.7%对15.1%）。
序贯培养基培养囊胚可提供更好的胚胎发育动态（第5天囊胚率为22%对13.6%）。两组的抱婴率无统计学显著差异（分别为18例对12例）。
