Dinan L, Bourne P, Whiting P
Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter, Devon, EX4 4PS, UK.
Phytochem Anal. 2001 Mar-Apr;12(2):110-9. doi: 10.1002/pca.566.
Procedures are presented for the assessment of the phytoecdysteroid profiles in small plant samples (ca. 25 mg), using seeds of Sida spp. as an example. The procedures are suitable for the analysis of minute or valuable samples and provide copious information for chemotaxonomic purposes. Methanolic extracts of the plant material, after partitioning against hexane, were separated by reversed-phase gradient HPLC monitored by PAD, RIA and bioassay. Aliquots of the fractions were also treated with Helix pomatia hydrolases, followed by RIA and bioassay, in order to assess the presence of hydrolysable ecdysteroid conjugates. Further information could also be obtained by separation of samples using normal-phase gradient HPLC. Among 11 species of Sida examined, seed extracts of S. acuta (= S. carpinifolia) and S. rhombifolia were found to contain significant amounts of ecdysteroids, seed extracts of S. filicaulis contained only moderate levels, whilst the remaining species showed no detectable levels of ecdysteroids. The ecdysteroid profiles of the extracts of the three positive species were significantly different, demonstrating that phytoecdysteroids have chemotaxonomic value in this genus.
本文介绍了以黄花稔属植物种子为例，对小植物样本（约25毫克）中的植物蜕皮甾体谱进行评估的方法。这些方法适用于微量或珍贵样本的分析，并为化学分类学目的提供丰富信息。植物材料的甲醇提取物经正己烷分配后，通过PAD、RIA和生物测定监测的反相梯度HPLC进行分离。各馏分的等分试样还用罗马蜗牛水解酶处理，然后进行RIA和生物测定，以评估可水解蜕皮甾体缀合物的存在。使用正相梯度HPLC分离样品也可获得更多信息。在所检测的11种黄花稔属植物中，尖叶黄花稔（= 心叶黄花稔）和菱叶黄花稔的种子提取物含有大量蜕皮甾体，丝状黄花稔的种子提取物仅含有中等水平的蜕皮甾体，而其余物种未检测到蜕皮甾体。这三种阳性物种提取物的蜕皮甾体谱有显著差异，表明植物蜕皮甾体在该属中具有化学分类学价值。
