Dinan L, Sarker S D, Bourne P, Whiting P, Sik V, Rees H H
Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Devon, U.K.
Arch Insect Biochem Physiol. 1999;41(1):18-23. doi: 10.1002/(SICI)1520-6327(1999)41:1<18::AID-ARCH4>3.0.CO;2-T.
Seeds of Rhagodia baccata afforded, in addition to 20-hydroxy-ecdysone and polypodine B, a novel phytoecdysteroid, (20R)-22-deoxy-20,21-dihydroxyecdysone, the structure of which was elucidated unequivocally by UV, LSIMS and NMR techniques. This compound possessed agonistic activity in the Drosophila melanogaster BII cell bioassay, with an ED50 value of 2.0 x 10(-7)M (ED50 value for 20-hydroxyecdysone = 7.5 x 10(-9)M). The distribution of ecdysteroids in plants of R. baccata has been determined. Highest levels are associated with the youngest aerial tissues and with the roots. Ecdysteroid profiles are qualitatively very similar throughout the plant, with 20-hydroxyecdysone and polypodine B predominating in all plant parts tested.
浆果藜的种子除了含有20-羟基蜕皮激素和多足素B外，还提供了一种新型植物蜕皮甾体，即(20R)-22-脱氧-20,21-二羟基蜕皮激素，其结构通过紫外光谱、低分辨离子回旋共振质谱和核磁共振技术得到了明确阐释。该化合物在黑腹果蝇BII细胞生物测定中具有激动活性，半数有效浓度（ED50）值为2.0×10⁻⁷M（20-羟基蜕皮激素的ED50值为7.5×10⁻⁹M）。已测定了浆果藜植物中蜕皮甾体的分布情况。最高含量与最幼嫩的地上组织和根部相关。在整株植物中，蜕皮甾体谱在质量上非常相似，在所有测试的植物部位中，20-羟基蜕皮激素和多足素B占主导地位。
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