Bóscolo F N, de Almeida S M, Cury J A, Lourenço R M
Faculdade de Odontologia de Piracicaba da UNICAMP.
Pesqui Odontol Bras. 2001 Jul-Sep;15(3):223-8. doi: 10.1590/s1517-74912001000300008.
The present study evaluated the effect of low doses of electron radiation on the activity of phosphodiesterases in granulation tissue. In order to induce growth of granulation tissue, a PVC sponge disk was introduced under the dorsal skin of 84 Wistar rats. The rats were divided in two groups, control and irradiated. The enzymatic activity was evaluated according to the evolution of the granulation tissue after 5, 7, 10, 14, 17, 20 and 24 days. Irradiation was carried out 3 days after the implantation of the sponge, by means of a linear accelerator, with energy of 6 MeV, and dose of 1.0 Gy. The results of this study showed that 5'-nucleotidase and ATPase had their activity directly affected by irradiation only in the beginning of the tissue repairing process. Alkaline phosphatase did not suffer any direct effect of irradiation. It is possible that the main factor has been the damage of the cellular components responsible for the growth of granulation tissue, which determine the production of enzymes according to the necessity.
本研究评估了低剂量电子辐射对肉芽组织中磷酸二酯酶活性的影响。为诱导肉芽组织生长，将聚氯乙烯海绵盘植入84只Wistar大鼠的背部皮肤下。大鼠分为两组，对照组和辐照组。根据植入后5、7、10、14、17、20和24天肉芽组织的生长情况评估酶活性。在植入海绵3天后，通过直线加速器进行辐照，能量为6兆电子伏特，剂量为1.0戈瑞。本研究结果表明，仅在组织修复过程开始时，5'-核苷酸酶和ATP酶的活性受到辐照的直接影响。碱性磷酸酶未受到辐照的任何直接影响。可能的主要因素是负责肉芽组织生长的细胞成分受损，这些细胞成分根据需要决定酶的产生。
