de Almeida Solange Maria, da Cruz Adriana Dibo, Ferreira Rívea Inês, Vizioli Mário Roberto, Bóscolo Frab Norberto
Department of Oral Diagnosis, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Braz Dent J. 2007;18(3):208-14. doi: 10.1590/s0103-64402007000300006.
The aim of this study was to assess the effect of low-dose electron irradiation on morphological features of the wound healing process in rat skin. Surgical wounds were inflicted with a 2.3 x 1.4 cm template on 84 male rats that were assigned to 4 groups: IG1, immediately irradiated; IG2, irradiated 3 days after inflicting the wound; CG1 and CG2, control groups. Rats in IG1 and IG2 groups had their wounds exposed to 1 Gy of 6 MeV electron beam radiation, immediately after surgery and on the third postoperative day, respectively. Qualitative and histophotometric evaluations of tissue repair structures were carried out. Data were analyzed by ANOVA and Tukey's test (alpha = 0.05) and regression analysis. The repair process was delayed since the first sacrifice time in both irradiated groups, but in IG1, wound healing was closer to that of CG1; whereas in IG2, the delay was more pronounced. Based on the histological findings, it is possible to conclude that a low-dose of electron radiation delayed tissue repair in rat skin. The delay was longer in the skin irradiated 3 days after the beginning of tissue repair. However, the low-energy electron irradiation did not prevent wound healing.
本研究的目的是评估低剂量电子辐照对大鼠皮肤伤口愈合过程形态学特征的影响。在84只雄性大鼠身上用2.3×1.4厘米的模板造成手术伤口，并将其分为4组：IG1组，立即辐照；IG2组，伤口造成后3天辐照；CG1组和CG2组为对照组。IG1组和IG2组的大鼠分别在手术后立即和术后第3天，将其伤口暴露于1 Gy的6 MeV电子束辐射下。对组织修复结构进行了定性和组织光度学评估。数据采用方差分析、Tukey检验（α = 0.05）和回归分析。在两个辐照组中，自首次处死时间起修复过程就延迟了，但在IG1组中，伤口愈合更接近CG1组；而在IG2组中，延迟更为明显。根据组织学结果，可以得出结论，低剂量电子辐射会延迟大鼠皮肤的组织修复。在组织修复开始3天后辐照的皮肤中，延迟时间更长。然而，低能量电子辐照并未阻止伤口愈合。
