用十六烷基三甲基溴化铵通过共振光散射测量法测定DNA。
Determination of DNA with cetyltrimethylammonium bromide by the measurement of Resonance Light Scattering.
作者信息
Li Y F, Shu W Q, Feng P, Huang C Z, Li M
机构信息
Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, P. R. China.
出版信息
Anal Sci. 2001 Jun;17(6):693-6. doi: 10.2116/analsci.17.693.
A simple assay of DNA was developed based on the measurements of enhanced signals of Resonance Light Scattering (RLS) of cetyltrimethylammonium bromide (CTMAB) by DNA. The enhanced RLS signals, measured by simultaneously scanning the excitation and emission monochromators of a common spectrofluorometer with lambda ex = lambda em, was optimized for the DNA assay with CTMAB. On the conditions of pH 2.21 and ionic strength 0.002, the enhanced RLS intensity at 470.0 nm, delta I, was found to be proportional to the concentration of DNA in the range 0-2.5 micrograms/ml if 1.5 x 10(-5) M CTMAB was used. Limits of determination for calf thymus DNA and fish sperm DNA were 4.9 ng/ml and 9.2 ng/ml, respectively. Synthetic samples were determined with the recovery ratio ranging from 93.2% to 105.1%, and the RSD is lower than 2.7%.
基于DNA对十六烷基三甲基溴化铵（CTMAB）共振光散射（RLS）增强信号的测量，开发了一种简单的DNA检测方法。通过用常见的荧光分光光度计同时扫描激发和发射单色器（λex = λem）来测量增强的RLS信号，该信号针对使用CTMAB的DNA检测进行了优化。在pH 2.21和离子强度0.002的条件下，如果使用1.5×10⁻⁵ M CTMAB，发现在470.0 nm处增强的RLS强度ΔI与0至2.5微克/毫升范围内的DNA浓度成正比。小牛胸腺DNA和鱼精DNA的测定限分别为4.9纳克/毫升和9.2纳克/毫升。对合成样品进行测定，回收率在93.2%至105.1%之间，相对标准偏差低于2.7%。
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