Gao Dejiang, He Na, Tian Yuan, Chen Yanhua, Zhang Hanqi, Yu Aimin
College of Chemistry, Jilin University, Changchun, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Nov;68(3):573-7. doi: 10.1016/j.saa.2006.12.030. Epub 2006 Dec 22.
In this paper, the anionic surfactant sodium dodecylbenzene sulphonate (SDBS) and cationic surfactant cetyltrimethylammonium bromide (CTMAB) were used as resonance light scattering (RLS) probe to determine bovine serum albumin (BSA). Based on the weak RLS intensity of SDBS-CTMAB probe and the enhancement of RLS intensity of BSA in the presence of the probe, a simple assay for BSA was developed. The experimental results showed that the formation of three component complex BSA-SDBS-CTMAB is the main reason for the enhancement of RLS intensity of BSA, in which SDBS as a bridge can interact with both BSA and CTMAB. The effects of pH value, incubation time, concentrations of SDBS and CTMAB on the enhanced RLS intensity of BSA were investigated. Under the optimum conditions, the enhanced RLS intensity is proportional to the concentration of BSA in the range from 2.5 x 10(-8) to 2.0 x 10(-6)mol L(-1). The detection limit is 9.7 x 10(-9) mol L(-1) for BSA. The study of foreign substance effect on the determination of BSA indicated that most of metal ions have little effect on the determination of BSA. The results of assay for BSA in synthetic samples were satisfactory.
本文以阴离子表面活性剂十二烷基苯磺酸钠（SDBS）和阳离子表面活性剂十六烷基三甲基溴化铵（CTMAB）作为共振光散射（RLS）探针来测定牛血清白蛋白（BSA）。基于SDBS - CTMAB探针的弱RLS强度以及在该探针存在下BSA的RLS强度增强，建立了一种简单的BSA测定方法。实验结果表明，三元复合物BSA - SDBS - CTMAB的形成是BSA的RLS强度增强的主要原因，其中SDBS作为桥梁可同时与BSA和CTMAB相互作用。研究了pH值、孵育时间、SDBS和CTMAB浓度对BSA增强的RLS强度的影响。在最佳条件下，增强的RLS强度在2.5×10⁻⁸至2.0×10⁻⁶mol·L⁻¹范围内与BSA浓度成正比。BSA的检测限为9.7×10⁻⁹mol·L⁻¹。对影响BSA测定的外来物质的研究表明，大多数金属离子对BSA的测定影响很小。合成样品中BSA的测定结果令人满意。
