Li Yongxin, Wu Yuqin, Chen Jinlong, Zhu Changqin, Wang Lun, Zhuo Sujuang, Zhao Danhua
College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, PR China.
Anal Bioanal Chem. 2003 Oct;377(4):675-80. doi: 10.1007/s00216-003-2160-2. Epub 2003 Aug 23.
On the basis of enhancement of resonance light scattering (RLS) of copper phthalocyanine tetrasulfonic acid (CuTSPc) by nucleic acids and cetyltrimethylammonium bromide (CTMAB) under suitable conditions, a new RLS method for determination of nucleic acids in aqueous solutions has been developed. At pH 9.80-10.95 and ionic strength 0.01 mol L(-1) (NaCl), the interaction of copper phthalocyanine tetrasulfonic acid with nucleic acids in the presence of cetyltrimethylammonium bromide results in enhanced RLS signals at 282.0 nm, 383.6 nm, and 616.2 nm in the enhanced regions. It was found that the enhanced RLS intensity at 383.6 nm was proportional to the concentration of nucleic acids within suitable ranges. The limits of detection were 10.6 ng mL(-1) for fish sperm DNA and 32.4 ng mL(-1) for calf thymus DNA when the concentration of copper phthalocyanine tetrasulfonic acid was 2.0 x 10(-6) mol L(-1). This method is rapid, simple and sensitive. In addition, the reagents used are relatively inexpensive, stable, and easily synthesised. The method can be applied to the determination of nucleic acids in the presence of coexisting substances, and we have applied it to the determination of DNA in synthetic samples, with satisfactory results.
基于核酸和十六烷基三甲基溴化铵（CTMAB）在合适条件下对四磺酸铜酞菁（CuTSPc）共振光散射（RLS）的增强作用，建立了一种测定水溶液中核酸的新RLS方法。在pH 9.80 - 10.95和离子强度0.01 mol L⁻¹（NaCl）条件下，四磺酸铜酞菁与核酸在十六烷基三甲基溴化铵存在下相互作用，在增强区域282.0 nm、383.6 nm和616.2 nm处产生增强的RLS信号。发现在合适范围内，383.6 nm处增强的RLS强度与核酸浓度成正比。当四磺酸铜酞菁浓度为2.0×10⁻⁶ mol L⁻¹时，鱼精DNA的检测限为10.6 ng mL⁻¹，小牛胸腺DNA的检测限为32.4 ng mL⁻¹。该方法快速、简单且灵敏。此外，所用试剂相对便宜、稳定且易于合成。该方法可用于共存物质存在下核酸的测定，我们已将其应用于合成样品中DNA的测定，结果令人满意。
