Yang B, Kotani A, Arai K, Kusu F
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, 100700, China.
Anal Sci. 2001 May;17(5):599-604. doi: 10.2116/analsci.17.599.
A simple electrochemical method for estimating the antioxidant activity (AA) of flavonoids has been developed. The proposed method is based on a measurement of the half-wave potential (E1/2) of the first oxidation wave of flavonoids by using flow-through column electrolysis. At the same time, the lipid peroxidation (LPO) inhibiting effects of these flavonoids were determined. A quantitative structure-activity relationship was obtained to describe the AA of flavonoids: IC50(microM) = 30.36 + 151.50 E1/2 (V) -12.63 log P (r = 0.852), where IC50 represents the concentration for 50% inhibition of LPO, and P represents the octanol/water partition coefficient. This method is expected to be useful for the quick screening of flavonoid antioxidants, and evaluating the AA of flavonoid-containing foods and medicinal plants.
已开发出一种用于估算黄酮类化合物抗氧化活性（AA）的简单电化学方法。所提出的方法基于使用流通柱电解法测量黄酮类化合物第一氧化波的半波电位（E1/2）。同时，测定了这些黄酮类化合物对脂质过氧化（LPO）的抑制作用。获得了定量构效关系以描述黄酮类化合物的抗氧化活性：IC50（微摩尔）= 30.36 + 151.50 E1/2（伏）- 12.63 log P（r = 0.852），其中IC50表示抑制50%脂质过氧化的浓度，P表示正辛醇/水分配系数。该方法有望用于黄酮类抗氧化剂的快速筛选，以及评估含黄酮类食物和药用植物的抗氧化活性。
