Machovich R
Biochim Biophys Acta. 1975 Jul 21;400(1):62-8. doi: 10.1016/0005-2795(75)90126-9.
Two forms of thrombin (Ts-thrombin and Tp-thrombin) were found with respect to heparin sensitivity. Inactivation of Ts-thrombin by antithrombin-III was facilitated with heparin, whereas inactivation of Tp-thrombin was not. Both thrombins were bound to heparin in a Sephadex G-200 gel filtration experiment. Ts-thrombin proved to be more stable and was better protected by heparin against heat inactivation of 54 degrees C than Tp-thrombin.
关于肝素敏感性，发现了两种形式的凝血酶（Ts-凝血酶和Tp-凝血酶）。抗凝血酶III对Ts-凝血酶的灭活作用在肝素存在时会加速，而对Tp-凝血酶则不会。在葡聚糖凝胶G-200凝胶过滤实验中，两种凝血酶均与肝素结合。结果证明，Ts-凝血酶比Tp-凝血酶更稳定，并且在54摄氏度的热灭活过程中，肝素对其保护作用更好。
