抗凝血酶III、α2-巨球蛋白和α1-蛋白酶抑制剂对α-和β-凝血酶的灭活作用。
Inactivation of alpha- and beta-thrombin by antithrombin-III, alpha 2-macroglobulin and alpha 1-proteinase inhibitor.
作者信息
Machovich R, Borsodi A, Blaskó G, Orakzai S A
出版信息
Biochem J. 1977 Nov 1;167(2):393-8. doi: 10.1042/bj1670393.
Inactivation of alpha- and beta-thrombin by alpha 2-macroglobulin, by alpha 1-proteinase inhibitor and by antithrombin-III and heparin was studied. The amount of alpha- and beta-thrombin inactivated by antithrombin-III was proportional to the concentration of the inhibitor, but the inactivation rates of the two forms of thrombin were different. Heparin facilitated complex-formation between alpha-thrombin and antithrombin-III, whereas inactivation of beta-thrombin by antithrombin was only slightly influenced, even at a heparin concentration two orders of magnitude higher. alpha 2-Macroglobulin inhibited both alpha- and beta-thrombin activity similarly, i.e. the amount of alpha- and beta-thrombin inactivated as well as the rates of their inhibition were the same. alpha 1-Proteinase inhibitor also formed a complex with alpha- and beta-thrombin, similarly to antithrombin-III, although the inactivation of the enzyme needed high inhibitor concentration and long incubation time. These results suggest that the inactivation of beta-thrombin, if it occurs in the plasma, is also controlled by plasma inhibitors.
研究了α2-巨球蛋白、α1-蛋白酶抑制剂、抗凝血酶III和肝素对α-凝血酶和β-凝血酶的灭活作用。抗凝血酶III灭活α-凝血酶和β-凝血酶的量与抑制剂浓度成正比，但两种形式凝血酶的灭活速率不同。肝素促进α-凝血酶与抗凝血酶III之间形成复合物，而即使在肝素浓度高两个数量级的情况下，抗凝血酶对β-凝血酶的灭活也仅受到轻微影响。α2-巨球蛋白对α-凝血酶和β-凝血酶活性的抑制作用相似，即α-凝血酶和β-凝血酶被灭活的量及其抑制速率相同。α1-蛋白酶抑制剂也与α-凝血酶和β-凝血酶形成复合物，与抗凝血酶III类似，尽管该酶的灭活需要高抑制剂浓度和长时间孵育。这些结果表明，如果β-凝血酶在血浆中发生灭活，其也受血浆抑制剂的控制。
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