电刺激对人皮肤成纤维细胞和U937人单核细胞系转化生长因子-β1分泌的影响

Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line.

作者信息

Todd I, Clothier R H, Huggins M L, Patel N, Searle K C, Jeyarajah S, Pradel L, Lacey K L

机构信息

Division of Molecular and Clinical Immunology, School of Clinical Laboratory Sciences, University of Nottingham Faculty of Medicine and Health Sciences, Queen's Medical Centre, Nottingham NG7 2UH, UK.

出版信息

Altern Lab Anim. 2001 Nov-Dec;29(6):693-701. doi: 10.1177/026119290102900611.

DOI:10.1177/026119290102900611

PMID:11709043

Abstract

The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. The growth and viability of growing and confluent dermal fibroblasts were not directly influenced by the separate microcurrent phases. One form of microcurrent (designated phase 1) stimulated both dermal fibroblasts and U937 cells to secrete transforming growth factor-beta 1 (TGF-beta 1), which is an important regulator of cell-mediated inflammation and tissue regeneration, but none of the three phases stimulated secretion of the pro-inflammatory cytokine interleukin-6 by U937 cells. The stimulation of TGF-beta 1 secretion in these experiments was not dramatic (a median increase over control levels of 20-30%), although it could be biologically significant.

摘要

研究了ACE刺激器产生的三个阶段微电流对人皮肤成纤维细胞和U937人单核细胞系的体外作用。生长中的和汇合的皮肤成纤维细胞的生长和活力不受单独微电流阶段的直接影响。一种微电流形式（指定为阶段1）刺激皮肤成纤维细胞和U937细胞分泌转化生长因子-β1（TGF-β1），TGF-β1是细胞介导的炎症和组织再生的重要调节因子，但三个阶段均未刺激U937细胞分泌促炎细胞因子白细胞介素-6。在这些实验中，TGF-β1分泌的刺激并不显著（比对照水平中位数增加20%-30%），尽管它可能具有生物学意义。