Casal S, Macedo B, Oliveira M B
CEQUP/Serviço de Bromatologia, Faculdade de Farmácia do Porto, Portugal.
J Chromatogr B Biomed Sci Appl. 2001 Nov 5;763(1-2):1-8. doi: 10.1016/s0378-4347(01)00349-8.
A method is described for evaluation of fat-soluble vitamin in human adipose tissue with the aim to obtain, accurately and within the shortest analysis time, a time-integrated measure of exposure to vitamins from the diet. Fat tissue was deproteinized with ethanol and extracted with n-hexane. Normal-phase HPLC was performed in a Lichrosorb Si60 column with a gradient of n-hexane-2-propanol at 1 ml/min. Detection was accomplished using a diode-array system (for retinol and beta-carotene) in series with a fluorescence detector (alpha-tocopherol). The method was validated and applied to human adipose tissue in a total of 140 subjects. The mean contents found were 0.43, 0.84, 240.3 microg/g for retinol, beta-carotene and alpha-tocopherol, respectively. The method is sensitive enough for detecting the compounds in 1.6 mg of adipose tissue considering the lowest concentration found.
描述了一种评估人体脂肪组织中脂溶性维生素的方法，目的是在最短的分析时间内准确获得饮食中维生素暴露的时间积分测量值。脂肪组织用乙醇脱蛋白，并用正己烷萃取。在Lichrosorb Si60柱上进行正相高效液相色谱，以1 ml/min的流速用正己烷 - 2 - 丙醇梯度洗脱。使用二极管阵列系统（用于视黄醇和β - 胡萝卜素）与荧光检测器（α - 生育酚）串联进行检测。该方法经过验证并应用于总共140名受试者的人体脂肪组织。发现的视黄醇、β - 胡萝卜素和α - 生育酚的平均含量分别为0.43、0.84、240.3 μg/g。考虑到所发现的最低浓度，该方法对于检测1.6 mg脂肪组织中的化合物足够灵敏。
