Zou Xiao-li, Zeng Hong-yan, Li Yuan-qian, Li Xiao-xia, Zhang Si-wei, Zheng Bo, Huang Cheng-yu
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Sep;38(5):879-81.
To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.
Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.
The linear ranges for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL-500 microg/mL, 0.030 microg/mL-500 microg/mL, 0.12 microg/mL-500 microg/mL, and 0.015 microg/mL-500 microg/mL, respectively. The detection limits for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL, 0.030 microg/mL, 0.12 microg/mL and 0.015 microg/mL, respectively. The relative standard derivations (RSD) for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.75%, 0.54%, 2.06% and 2.74%, respectively. The proposed method recovered 92%-116%, 98%-112%, 84.8%-106%, and 90%-105% retinol, VD3, alpha-tocopherol and beta-carotene in human serums respectively.
The method is simple, quick and applicable to all of the four fat-solutable vitamins.
