Sunaga H, Fujieda S, Tsuzuki H, Asamoto K, Fukuda M, Saito H
Department of Otorhinolaryngology, Fukui Medical University, Japan.
Anticancer Res. 2001 Jul-Aug;21(4B):2901-6.
Epithelial hyperplasia and dysplasia have been diagnosed as precancerous lesions and have been discussed in relationship to carcinogenesis. We analyzed the immunohistochemical expression of granulocyte colony-stimulating factor receptor (G-CSFR) and platelet-derived endothelial cell growth factor (PD-ECGF) in oral and oropharynx; 33 samples of normal epithelium, 28 samples of hyperplasia, 16 samples of dysplasia and 58 samples of squamous cell carcinoma. Also, we examined mean vessel density (MVD) by using CD34 staining and proliferating cell nuclear antigen (PCNA) staining. Dysplasia and head and neck Squamous Cell Carcinoma (SCC) exhibited higher G-CSFR expression and MVD than normal or hyperplastic epithelium (p <0.01). In the PD-ECGF staining, significant differences were found between SCC and normal epithelium, hyperplasia and dysplasia (p<0.001). In dysplasia and hyperplasia, PD-ECGF expression was significantly correlated with PCNA expression (r=0.345, p=0.025), however it was not correlated with the MVD. G-CSFR expression was not correlated with either PCNA or MVD. These results suggest that G-CSFR and PD-ECGF might be concerned with different carcinogenesis pathways of the squamous cells in the oral region and that PD-ECGF may be concerned with epithelial proliferation rather than angiogenesis.
上皮增生和发育异常已被诊断为癌前病变，并已在与致癌作用的关系中进行了讨论。我们分析了粒细胞集落刺激因子受体（G-CSFR）和血小板衍生内皮细胞生长因子（PD-ECGF）在口腔和口咽中的免疫组化表达；33例正常上皮样本、28例增生样本、16例发育异常样本和58例鳞状细胞癌样本。此外，我们通过使用CD34染色和增殖细胞核抗原（PCNA）染色来检测平均血管密度（MVD）。发育异常和头颈部鳞状细胞癌（SCC）的G-CSFR表达和MVD高于正常或增生上皮（p<0.01）。在PD-ECGF染色中，SCC与正常上皮、增生和发育异常之间存在显著差异（p<0.001）。在发育异常和增生中，PD-ECGF表达与PCNA表达显著相关（r=0.345，p=0.025），然而它与MVD不相关。G-CSFR表达与PCNA或MVD均不相关。这些结果表明，G-CSFR和PD-ECGF可能与口腔区域鳞状细胞的不同致癌途径有关，并且PD-ECGF可能与上皮增殖而非血管生成有关。
