Fujieda S, Sunaga H, Tsuzuki H, Tanaka N, Saito H
Department of Otorhinolaryngology, Fukui Medical University, Yoshida, Japan.
Clin Cancer Res. 1998 Jul;4(7):1583-90.
Platelet-derived endothelial cell growth factor (PD-ECGF) was isolated as an endothelial cell mitogen from platelets. In this study, we investigated the expression of PD-ECGF and counted microvessels in 58 oral and oropharyngeal squamous cell carcinoma (SCC) specimens by an immunohistochemical technique to examine their prognostic significance and performed tumor in vitro sensitivity to 5-fluorouracil (5-FU) and cisplatin as determined by a bioluminescence assay of the ATP values of tumor cells after continuous exposure. The percentage of PD-ECGF-positive tumor cells (PD-ECGF score) was correlated with the frequency of the recurrence of disease (P=0.0043) but not with sex, tumor size, metastasis, or clinical stage. Overall survival of the high PD-ECGF expression group (>40% PD-ECGF score) was shorter than the low expression (<40%) group (P=0.0365). Vessel count was correlated with lymph node metastasis and clinical stage. The survival of patients with hypervascularity (more than the median of intratumor vessel counts, >82) was shorter than that of those with hypovascularity (vessel count <81, P=0.0446). However, there was no association between PD-ECGF expression and vessel count. Cox proportional multivariate analysis showed that PD-ECGF expression was the most significant independent prognostic indicator for overall survival. The susceptibility to 5-FU cytotoxicity in the extremely high PD-ECGF expression groups (>70% of PD-ECGF score) was significantly higher than that in the low group, whereas there was no difference in their sensitivity to cisplatin. These results showed that carcinoma cells with high PD-ECGF expression were sensitive to 5-FU in spite of poor prognosis. These data provide further information when deciding on adjuvant therapy for oral and oropharyngeal SCCs.
血小板衍生的内皮细胞生长因子（PD - ECGF）最初是作为一种从血小板中分离出来的内皮细胞促有丝分裂原。在本研究中，我们采用免疫组织化学技术调查了58例口腔和口咽鳞状细胞癌（SCC）标本中PD - ECGF的表达情况并对微血管进行计数，以检验其预后意义；同时通过连续暴露后肿瘤细胞ATP值的生物发光测定法，检测了肿瘤对5 - 氟尿嘧啶（5 - FU）和顺铂的体外敏感性。PD - ECGF阳性肿瘤细胞的百分比（PD - ECGF评分）与疾病复发频率相关（P = 0.0043），但与性别、肿瘤大小、转移情况或临床分期无关。高PD - ECGF表达组（PD - ECGF评分>40%）的总生存期短于低表达组（<40%，P = 0.0365）。微血管计数与淋巴结转移及临床分期相关。血管丰富（肿瘤内血管计数超过中位数，>82）患者的生存期短于血管较少（血管计数<81，P = 0.0446）的患者。然而，PD - ECGF表达与血管计数之间并无关联。Cox比例多因素分析显示，PD - ECGF表达是总生存期最显著的独立预后指标。在PD - ECGF表达极高的组（PD - ECGF评分>70%）中，对5 - FU细胞毒性的敏感性显著高于低表达组，而它们对顺铂的敏感性则无差异。这些结果表明，尽管预后较差，但PD - ECGF高表达的癌细胞对5 - FU敏感。这些数据为决定口腔和口咽SCC的辅助治疗提供了更多信息。
