意大利犬细小病毒2型进化的证据。

Evidence for evolution of canine parvovirus type 2 in Italy.

作者信息

Buonavoglia Canio, Martella Vito, Pratelli Annamaria, Tempesta Maria, Cavalli Alessandra, Buonavoglia Domenico, Bozzo Giancarlo, Elia Gabriella, Decaro Nicola, Carmichael Leland

机构信息

Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.

Department of Pathology and Infectious Diseases, University of Messina, Messina, Italy2.

出版信息

J Gen Virol. 2001 Dec;82(Pt 12):3021-3025. doi: 10.1099/0022-1317-82-12-3021.

DOI:10.1099/0022-1317-82-12-3021

PMID:11714979

Abstract

Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay.

摘要

从患有严重出血性腹泻的犬只中分离出两株犬细小病毒（CPV）。通过单克隆抗体抗原分析和型特异性引物PCR鉴定，预测其具有2b型抗原特异性。然而，衣壳蛋白编码基因的序列分析显示有两个氨基酸变化。其中一个变化发生在病毒衣壳主要抗原位点的第426位（天冬氨酸变为谷氨酸），导致CPV 2b型特有的一个残基被取代。通过开发一种限制性片段长度多态性（RFLP）检测方法，克服了现有分型方法无法区分这种抗原变异体的问题。