意大利分离出的犬细小病毒毒株的抗原特性
Antigenic characterization of canine parvovirus strains isolated in Italy.
作者信息
Sagazio P, Tempesta M, Buonavoglia D, Cirone F, Buonavoglia C
机构信息
Dipartimento di Sanita', Patologia, Farmaco-Tossicologia e Benessere Animale, Facolta' di Medicina Veterinaria, Valenzano, Bari, Italy.
出版信息
J Virol Methods. 1998 Aug;73(2):197-200. doi: 10.1016/s0166-0934(98)00055-x.
28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b.
从意大利患有出血性肠胃炎的犬只中获取了28株犬细小病毒2型（CPV - 2）毒株。使用四种具有鉴别能力的单克隆抗体对CPV - 2毒株的抗原结构进行了表征。此外，还检测了四种疫苗株。与澳大利亚和英国的报道相似，观察到CPV - 2a（25/28株分离物）的流行率远高于另一种变异型CPV - 2b（3/28株分离物）。通过聚合酶链反应（PCR）扩增CPV - 2、CPV - 2a和CPV - 2b代表性毒株的DNA片段（2.2 kbp），并将产物用限制性内切酶（RE）RsaI、HpaII、HindIII和PvuII进行消化。RvaI酶可区分CPV - 2与CPV - 2a和CPV - 2b。
Zotero 插件