Gong S, Zeng X, Wang J
Union Hospital, Tongji Medical University, Wuhan 430022.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1998 Jun;33(3):158-60.
To understand the role of intercellular adhesion molecule-1(ICAM-1) in the immune response of the inner ear.
Inner ear immune response was induced in rats by inoculation of key-hole limpet hemocyanine(KLH) into the scala tympani of animals who had been systemically sensitized. Then the expression of ICAM-1 in the inner ear was examined by immunohistochemistry.
ICAM-1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules (CV) as early as 6 hours postchallenge. Expression of ICAM-1 was observed on the epithelium of the endolymphatic sac(ES) and perisaccular region at 12 hours. The intensity of ICAM-1 staining reached a maximum by 24 or 48 hours in these sites of the inner ear. By day 28, most specimens were devoid of significant staining for ICAM-1.
The study elucidates the important role of adhesion molecules in extravasation of inflammatory cells from the systemic circulation during an inner ear immune response. It also shows that cytokines controlling expression of adhesion molecules may be released by cells located outside of ES besides those cells in the ES.
了解细胞间黏附分子-1（ICAM-1）在内耳免疫反应中的作用。
对已全身致敏的动物，通过向鼓阶注射钥孔戚血蓝蛋白（KLH）诱导大鼠内耳免疫反应。然后采用免疫组化法检测内耳中ICAM-1的表达。
攻击后6小时，最早在螺旋蜗轴静脉（SMV）及其集合小静脉（CV）的上皮中发现ICAM-1。12小时时，在内淋巴囊（ES）及囊周区域的上皮中观察到ICAM-1的表达。在内耳这些部位，ICAM-1染色强度在24或48小时达到最大值。到第28天，大多数标本中ICAM-1无明显染色。
该研究阐明了黏附分子在内耳免疫反应期间炎症细胞从体循环渗出中的重要作用。研究还表明，除了ES中的细胞外，ES外的细胞也可能释放控制黏附分子表达的细胞因子。
