Zeng X, Gong S
Department of Otolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 1998 Nov;12(11):513-6.
During secondary inner ear immune responses against keyhole limpet hemocyanin (KLH), to understand the change of interleukin-6 (IL-6) levels in perilymph and serum, IL-6 levels in perilymph and serum were investigated using an immunoassay. No IL-6 levels were detectable at Day 0 in perilymph. The earliest perilymph IL-6 levels were observed after 6 h, peaking at 24 h, and then decreasing gradually. IL-6 level remnants were detected at 7 days in perilymph. In contrast, during this observation period, low IL-6 levels were only detectable in perilymph from control ears, disappearing after 72 h. IL-6 levels don't change in serum. Previous study has identified the endolymphatic sac (ES) lags behind the earlier appearance of intercellular adhesion molecule-1 (ICAM-1) in the spiral modiolar vein and spiral ligament during immune response. The present study not only provides further support for the existence of an inner ear immune response which is regulated by cytokines, but also supports that cytokines controlling expression of adhesion molecules are released probably by cells which located out of ES.
在针对钥孔戚血蓝蛋白（KLH）的内耳二次免疫反应期间，为了解外淋巴和血清中白细胞介素-6（IL-6）水平的变化，采用免疫分析法对外淋巴和血清中的IL-6水平进行了研究。外淋巴在第0天未检测到IL-6水平。最早在外淋巴中观察到IL-6水平是在6小时后，在24小时达到峰值，然后逐渐下降。在外淋巴中第7天检测到IL-6水平残留。相比之下，在此观察期内，仅在对照耳的外淋巴中可检测到低水平的IL-6，72小时后消失。血清中IL-6水平无变化。先前的研究已确定，在免疫反应期间，内淋巴囊（ES）在螺旋蜗轴静脉和螺旋韧带中细胞间黏附分子-1（ICAM-1）较早出现之后才出现。本研究不仅为细胞因子调节内耳免疫反应的存在提供了进一步支持，也支持控制黏附分子表达的细胞因子可能由ES外的细胞释放。
