Baou N, Bouras M, Droz J P, Dutrieux-Berger N, Bouvier R, Benahmed M, Krantic S
INSERM 407, Faculté de Médecine Lyon Sud, Oullins, France.
Cancer Detect Prev. 2001;25(5):446-53.
The expression of five (sst1-sst5) somatostatin (SRIF) receptor mRNAs was compared between normal and tumoral testicular samples diagnosed as either seminoma or non-seminoma. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that all testicular tissues studied (total of 24) contained sst5 receptor transcripts, whereas the sst2 was absent in all of them. In contrast to the normal tissue samples, both types of tumors (total of 12) did not contain sst4 transcripts. sst3 mRNA was expressed in normal and non-seminoma samples, but not in seminomas. sst1 transcripts were not found in normal and seminoma tissues. However, all studied non-seminomas contained this mRNA. Our data thus points to a specific pattern of SRIF receptor mRNA expression in each type of the samples analyzed. Moreover, they further indicate that the presence of sst1 and sst3 transcripts might be used as an additional criterion to distinguish between seminoma and nonseminoma tumors.
比较了正常睾丸样本与诊断为精原细胞瘤或非精原细胞瘤的肿瘤性睾丸样本中五种（sst1 - sst5）生长抑素（SRIF）受体mRNA的表达情况。逆转录聚合酶链反应（RT-PCR）分析表明，所有研究的睾丸组织（共24个）均含有sst5受体转录本，而所有组织中均不存在sst2。与正常组织样本相反，两种类型的肿瘤（共12个）均不含有sst4转录本。sst3 mRNA在正常和非精原细胞瘤样本中表达，但在精原细胞瘤中不表达。在正常和精原细胞瘤组织中未发现sst1转录本。然而，所有研究的非精原细胞瘤均含有该mRNA。因此，我们的数据表明在每种分析的样本类型中SRIF受体mRNA表达具有特定模式。此外，它们进一步表明sst1和sst3转录本的存在可作为区分精原细胞瘤和非精原细胞瘤肿瘤的额外标准。
