Owolabi A T, Proll E
Department of Biological Sciences, University of Calabar, Nigeria.
Acta Virol. 2001 Apr;45(2):73-9.
A virus inducing mosaic and severe leaf malformation, isolated from Senna hirsuta in Nigeria, was studied. The virus had a rather narrow host range, infecting a few species in Caesalpinaceae, Chenopodiaceae and Fabaceae families. The virus was widespread in southern Nigeria with prevalence ranging from 74% to 86.4% in some locations. It was transmitted mechanically and in a non-persistent manner by Myzuspersicae, Aphis craccivora and A. spiraecola. There was no evidence of transmission by seeds. Electron microscopy of leaf dip preparations revealed flexuous rod-shaped particles. The viral coat protein had Mr of 32.5 K. The virus reacted positively with a monoclonal antibody (MAb) to peanut stripe virus specific for potyviruses (members of the Potvvirus genus) and with antisera to turnip mosaic virus (TuMV), potato virus Y (PVY), TuMV, potato virus A (PVA), potato virus V (PVV) and bean yellow mosaic virus (BYMV), but it failed to react with antisera to celery mosaic virrus (CeMV), bean common mosaic virus (BCMV), soybean mosaic virus (SMV), and clover yellow mosaic virus (ClYMV) in plate-trapped ELISA (PTA-ELISA). No positive reaction was obtained when the virus was tested against any of the antisera in double-antibody sandwich ELISA (DAS-ELISA). This is the first report of natural infection of Senna species in Nigeria. The virus, tentatively designated as Senna mosaic virus (SeMV), seems to differ from other viruses previously described from Senna species in the literature and indeed other legume potyviruses in Nigeria.
对从尼日利亚的长柔毛番泻叶中分离出的一种引起花叶病和严重叶片畸形的病毒进行了研究。该病毒的寄主范围相当狭窄，仅感染苏木科、藜科和豆科的少数物种。该病毒在尼日利亚南部广泛传播，在一些地区的流行率为74%至86.4%。它通过桃蚜、花生蚜和绣线菊蚜以机械方式非持久性传播。没有种子传播的证据。叶片浸出液制剂的电子显微镜检查显示为弯曲的杆状颗粒。病毒外壳蛋白的分子量为32.5K。该病毒与针对马铃薯Y病毒属（马铃薯Y病毒属成员）的花生条纹病毒的单克隆抗体（MAb）以及与芜菁花叶病毒（TuMV）、马铃薯Y病毒（PVY）、TuMV、马铃薯A病毒（PVA）、马铃薯V病毒（PVV）和菜豆黄花叶病毒（BYMV）的抗血清呈阳性反应，但在平板捕获ELISA（PTA-ELISA）中，它与芹菜花叶病毒（CeMV）、菜豆普通花叶病毒（BCMV）、大豆花叶病毒（SMV）和三叶草黄花叶病毒（ClYMV）的抗血清不发生反应。在双抗体夹心ELISA（DAS-ELISA）中，当该病毒与任何一种抗血清进行检测时，均未获得阳性反应。这是尼日利亚长柔毛番泻叶自然感染的首次报道。该病毒暂定为长柔毛番泻叶花叶病毒（SeMV），似乎与文献中先前描述的来自长柔毛番泻叶的其他病毒以及尼日利亚的其他豆科马铃薯Y病毒不同。
